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Complementary Deoxyribonucleic Acid Cloning and Characterization of a Putative Human Axonemal Dynein Light Chain Gene¹

Department of Internal Medicine (K.K., W.E.T., R.S., M.G., C.E.F., S.B.), Division Of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University Of Medicine and Science, Los Angeles, California 90059; Karolinska Institute (S.A.), Stockholm, Sweden; and Department of Medical Genetics (P.J.C., P.V.H., G.V.C.), University of Antwerp, Antwerp B 2610, Belgium

Address all correspondence and requests for reprints to: Shalender Bhasin, M.D., Chief, Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, and Professor of Medicine, UCLA School of Medicine, 1621 E. 120^th Street, Los Angeles, California 90059.

Immotile Cilia Syndrome (ICS) is characterized by recurrent sinus and lung infections, bronchiectasis, and sperm immotility. Nasal cilia and sperm tails in patients with ICS exhibit a variety of ultrastructural defects, often including shortening or absence of the inner dynein arms. Immotile mutant strains of Chlamydomonas, a biflagellated algae, have ultrastructural defects similar to those seen in patients with this clinical disorder. Furthermore, splice-site mutations in the Chlamydomonas inner dynein arm gene (p28) are associated with impaired flagellar motility. We therefore hypothesized that the human homologue of the Clamydomonas dynein p28 gene would be an attractive candidate gene for patients with ICS. Accordingly, we cloned the full length complementary DNA (cDNA) and genomic clone by screening of appropriate libraries and databases, using the protein sequence of the Chlamydomonas p28 gene. The human homologue is encoded by a 921 bp transcript (accession no. AF006386) with an open reading frame of 257 amino acids. Using somatic cell and radiation hybrid panels, the hp28 gene was mapped to human chromosome 1p35.1. The hp28 cDNA probe hybridizes to sequences in all species on a zoo blot containing genomic DNA from yeast to human. Northern blot analysis reveals two hp28 gene transcripts, 0.9 and 2.5 kb, in many tissues. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5-kb transcript in the testis. The entire gene is included in a 20-kb EcoRI genomic fragment and has 7 exons and 6 introns. Cloning of the hp28 cDNA and mapping of the intron-exon junctions should now make it possible to test whether a subset of ICS is a consequence of mutations in the human axonemal dynein light chain gene hp28.

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