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Cattedra di Endocrinologia, Dipartimento di Medicina Sperimentale e Clinica (F.A., D.R., N.P., S.F.), Cattedra di Farmacologia, Facoltà di Farmacia (D.R.), Università di Reggio Calabria, 88100 Catanzaro, Italy, Cattedra di Endocrinologia, Ospedale Garibaldi, Università di Catania, Italy (D.G., A.I., R.V.)
Address correspondence and requests for reprints to: Sebastiano Filetti, M.D., Cattedra di Endocrinologia, Dipartimento di Medicina Sperimentale e Clinica, Via T. Campanella, 88100 Catanzaro, Italy.
Abstract
We report a PCR-based technique for detecting thyroid cancer metastases in small nodes <1.5 cm diameter by the amplification of thyroid specific transcripts TSH-receptor and thyroglobulin. A 100% correspondence with the histopathological diagnosis was observed in the 41/46 nodes (89%) in which an adequate sample was obtained at fine needle aspiration. The genetic analysis that resulted was more sensitive and accurate than both the cytological analysis (28% inadequate samples, 17% false negative diagnoses) and the thyroglobulin measurement in the aspirates (39% false negatives). The PCR-based genetic analysis may provide a useful tool for diagnosis and follow-up of thyroid cancer.
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