help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cudicini, C.
Right arrow Articles by Jégou, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cudicini, C.
Right arrow Articles by Jégou, B.
The Journal of Clinical Endocrinology & Metabolism Vol. 82, No. 5 1426-1433
Copyright © 1997 by The Endocrine Society

Experimental Studies

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -61

Corinne Cudicini2, Hervé Lejeune, Edith Gomez, Eugène Bosmans, François Ballet, José Saez and Bernard Jégou

Groupe D’Etude de la Reproduction Chez le Mâle (GERM) INSERM U. 435, Université de Rennes I, Campus de Beaulieu (C.C., E.G., B.J.), 35042 Rennes, Bretagne; Rhône-Poulenc Rorer, Département de la Sécurité du Médicament, Centre de Recherche de Vitry-Alfortville (C.C., F.B.), 94403 Vitry sur Seine Cedex; INSERM Institut National de Recherche Agronomique INRA U. 418, Hôpital Debrousse (H.L., J.S.), 69322 Lyon Cedex 05, France; and Eurogenetics (E.B.), 3980 Tessenderlo, Belgium

Address all correspondence and requests for reprints to: Bernard Jégou, Groupe d’Etude de la Reproduction Chez le Mâle INSERM U 435, Université de Rennes I, Campus de Beaulieu, 35042 Rennes, Bretagne, France. E-mail: bernard.jegou{at}univrennes1.fr

Interleukins (IL)-1 and -6 have been shown to be produced by several categories of cells in the rat testis and involved in the paracrine control of testicular function. Evidence of high amounts of IL-1 have been shown in the human testis, but nothing is known about its cellular origin. Furthermore, to our knowledge, the presence of IL-6 in the human testis has not yet been investigated. Therefore, the present study was aimed at identifying IL-1 and -6 expression and production within the human testis, using RT-PCR, bioassays, and enzyme linked immunosorbent assays. We demonstrated that IL-1 and -6 messenger RNA and proteins were produced constitutively in vitro by human Leydig cell- and Sertoli cell-enriched preparations. FSH only stimulated IL-6 production by Sertoli cell-enriched preparations, but increased the release of both IL-1 and -6 in germ cell-depleted Sertoli cell cultures. In addition, lipopolysaccharides and latex beads enhanced the production of both cytokines by Sertoli cell cultures, whereas human chorionic gonadotropin and lipopolysaccharides enhanced the release of both cytokines by Leydig cells. Enzyme linked immunosorbent assays and neutralization experiments revealed that human Sertoli cells produce essentially the {alpha} form of IL-1, whereas both forms, {alpha} and ß, are present in Leydig cells.

The demonstration that human Leydig and Sertoli cells produce IL-1 and -6 under the control of gonadotropin hormones and exogenous factors, opens the possibility to study the involvement of these cytokines in the control of testis function, in normal and pathological conditions in men. 82:




This article has been cited by other articles:


Home page
 Hum ReprodHome page
A. Le Tortorec, H. Denis, A-P. Satie, J-J. Patard, A. Ruffault, B. Jegou, and N. Dejucq-Rainsford
Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation
Hum. Reprod., June 20, 2008; (2008) den207v1.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
O. Sarkar, P. P. Mathur, C. Y. Cheng, and D. D. Mruk
Interleukin 1 Alpha (IL1A) Is a Novel Regulator of the Blood-Testis Barrier in the Rat
Biol Reprod, March 1, 2008; 78(3): 445 - 454.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
M. Fraczek and M. Kurpisz
Inflammatory mediators exert toxic effects of oxidative stress on human spermatozoa
J Androl, March 1, 2007; 28(2): 325 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Shchors, E. Shchors, F. Rostker, E. R. Lawlor, L. Brown-Swigart, and G. I. Evan
The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta
Genes & Dev., September 15, 2006; 20(18): 2527 - 2538.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Z.-M. Cai, Y.-T. Gui, X. Guo, J. Yu, L.-D. Guo, L.-B. Zhang, H. Wang, and J. Yu
Low Expression of Glycoprotein Subunit 130 in Ejaculated Spermatozoa from Asthenozoospermic Men
J Androl, September 1, 2006; 27(5): 645 - 652.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Ishikawa, K. Hwang, D. Lazzarino, and P. L. Morris
Sertoli Cell Expression of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer 1 and 5 Domain-Containing Proteins and Sterol Regulatory Element Binding Protein-1 Are Interleukin-1{beta} Regulated by Activation of c-Jun N-Terminal Kinase and Cyclooxygenase-2 and Cytokine Induction
Endocrinology, December 1, 2005; 146(12): 5100 - 5111.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. K. O'Bryan, O. Gerdprasert, D. J. Nikolic-Paterson, A. Meinhardt, J. A. Muir, L. M. Foulds, D. J. Phillips, D. M. de Kretser, and M. P. Hedger
Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1744 - R1755.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
Y Okuma, K Saito, A E O'Connor, D J Phillips, D M de Kretser, and M P Hedger
Reciprocal regulation of activin A and inhibin B by interleukin-1 (IL-1) and follicle-stimulating hormone (FSH) in rat Sertoli cells in vitro
J. Endocrinol., April 1, 2005; 185(1): 99 - 110.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Le Goffic, T. Mouchel, A. Ruffault, J.-J. Patard, B. Jegou, and M. Samson
Mumps Virus Decreases Testosterone Production and Gamma Interferon-Induced Protein 10 Secretion by Human Leydig Cells
J. Virol., March 1, 2003; 77(5): 3297 - 3300.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Oyhenart, R. Le Goffic, M. Samson, B. Jegou, and N. Raich
Phtf1 Is an Integral Membrane Protein Localized in an Endoplasmic Reticulum Domain in Maturing Male Germ Cells
Biol Reprod, March 1, 2003; 68(3): 1044 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Walch and P. L. Morris
Cyclooxygenase 2 Pathway Mediates IL-1{beta} Regulation of IL-1{alpha}, -1{beta}, and IL-6 mRNA Levels in Leydig Cell Progenitors
Endocrinology, September 1, 2002; 143(9): 3276 - 3283.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Le Goffic, T. Mouchel, F. Aubry, J.-J. Patard, A. Ruffault, B. Jegou, and M. Samson
Production of the Chemokines Monocyte Chemotactic Protein-1, Regulated on Activation Normal T Cell Expressed and Secreted Protein, Growth-Related Oncogene, and Interferon-{gamma}-Inducible Protein-10 Is Induced by the Sendai Virus in Human and Rat Testicular Cells
Endocrinology, April 1, 2002; 143(4): 1434 - 1440.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Morales-Montor, S. Baig, R. Mitchell, K. Deway, C. Hallal-Calleros, and R. T. Damian
Immunoendocrine Interactions During Chronic Cysticercosis Determine Male Mouse Feminization: Role of IL-6
J. Immunol., October 15, 2001; 167(8): 4527 - 4533.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Emiliani, M. Van den Bergh, A.-S. Vannin, J. Biramane, M. Verdoodt, and Y. Englert
Increased sperm motility after in-vitro culture of testicular biopsies from obstructive azoospermic patients results in better post-thaw recovery rate
Hum. Reprod., November 1, 2000; 15(11): 2371 - 2374.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel
Induction of Interleukin-1{alpha} Production in Murine Sertoli Cells by Interleukin-1
Biol Reprod, May 1, 2000; 62(5): 1291 - 1296.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
F. Aubry, C. Habasque, A.-P. Satie, B. Jégou, and M. Samson
Expression and Regulation of the CC-Chemokine Monocyte Chemoattractant Protein-1 in Rat Testicular Cells in Primary Culture
Biol Reprod, May 1, 2000; 62(5): 1427 - 1435.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, and M. Huleihel
Interleukin-1 Receptor Antagonist Is Produced by Sertoli Cells in Vitro
Endocrinology, April 1, 2000; 141(4): 1521 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. D. Mruk and C. Y. Cheng
Sertolin Is a Novel Gene Marker of Cell-Cell Interactions in the Rat Testis
J. Biol. Chem., September 17, 1999; 274(38): 27056 - 27068.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. P. De Miguel, J. Regadera, F. Martinez-Garcia, M. Nistal, and R. Paniagua
Oncostatin M in the Normal Human Testis and Several Testicular Disorders
J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 768 - 774.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
M. José de los Santos, D. J. Anderson, C. Racowsky, C. Simón, and J. A. Hill
Expression of Interleukin-1 System Genes in Human Gametes
Biol Reprod, December 1, 1998; 59(6): 1419 - 1424.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
D. Mruk, C.-H. Cheng, Y.-H. Cheng, M.-y. Mo, J. Grima, B. Silvestrini, W. M. Lee, and C. Y. Cheng
Rat Testicular Extracellular Superoxide Dismutase: Its Purification, Cellular Distribution, and Regulation
Biol Reprod, August 1, 1998; 59(2): 298 - 308.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. K. Fried, D. A. Bunkin, and A. S. Greenberg
Omental and Subcutaneous Adipose Tissues of Obese Subjects Release Interleukin-6: Depot Difference and Regulation by Glucocorticoid
J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 847 - 850.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society