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Adipose Tissue Lipolysis and Hormone-Sensitive Lipase Expression during Very-Low-Calorie Diet in Obese Female Identical Twins¹

Obesity Unit, Fourth Department of Medicine (V.S., M.K., V.H.), Charles University, Prague 2, Czech Republic; Laboratoire des Adaptations de l’Organisme à l’Exercice Musculaire, Service d’Exploration de la Fonction Respiratoire et de Médecine du Sport, Hôpital Purpan, and INSERM Unité 317, Institut Louis Bugnard, Hôpital Rangueil, Faculté de Médecine (I.H., I.G., F.C., M.B., M.D., D.R., M.G., M.L., D.L.), Université Paul Sabatier, 31403 Toulouse Cédex 4, France; and Department of Cell and Molecular Biology (C.H.), Lund University, 22100 Lund, Sweden

Address all correspondence and requests for reprints to: Dr. Dominique Langin, Institut National de la Santé et de la Recherche Médicale U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, F-31403 Toulouse Cédex 4, France. E-mail: langin{at}rangueil.inserm.fr

Eight pairs of obese female monozygotic twins were subjected to a 4-week, very-low-calorie diet (VLCD) that induced a decrease in mean body mass index from 32.9 ± 1.1 to 29.7 ± 1.1 kg/m². Infusion of the ß-adrenergic agonist, isoproterenol, induced an increase in plasma levels of nonesterified fatty acids and glycerol that was more pronounced during than before VLCD. sc fat biopsies were obtained before and during VLCD to study adipocyte lipolysis. ß-adrenergic sensitivity was moderately improved during VLCD. Basal and stimulated lipolyses, and hormone-sensitive lipase activity and protein levels were increased during VLCD. Before VLCD, intrapair resemblance was found for basal and stimulated lipolysis rates. In response to the treatment, intrapair resemblance was observed for basal lipolysis and for lipolysis stimulated with agents acting on plasma membrane receptors. These results suggest that the increase of basal lipolysis during VLCD is caused by an increase of hormone-sensitive lipase expression. They support the notion that the genotype may play a role in regulating the changes of adipose tissue lipolysis rates observed during VLCD.

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