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Cytokine Production by a New Undifferentiated Human Thyroid Carcinoma Cell Line, FB-1¹

Dipartimento di Oncologia (L.F., L.E.P., G.F., R.G., F.B.), Istituto di Endocrinologia (F.P.), Dipartimento di Chirurgia (P.M.), Universita’ degli Studi di Pisa, Pisa, 56100, Italy; Laboratorio di Microbiologia, II Facolta’ di Medicina e Chirurgia Varese, Universita’ degli Studi di Pavia (R.C., A.T.), Varese, 21100, Italy; Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita’ degli Studi di Napoli (M.T.B.), Napoli, 80100, Italy; and Dipartimento di Medicina Sperimentale e Clinica, Facolta’ di Medicina e Chirurgia di Catanzaro, Universita’ degli Studi di Reggio Calabria (A.F.), Catanzaro, 88100, Italy

Address all correspondence and requests for reprints to: Fulvio Basolo, M.D., Department of Oncology, University of Pisa, 57, Via Roma, 56126, Pisa, Italy.

A human anaplastic thyroid cancer cell line FB-1, derived from a 68-yr-old woman who underwent surgery for anaplastic thyroid cancer, has been established. The spindlelike cells have been proliferating stably for more than 2 yr. Karyotype analysis shows many abnormalities and many marker chromosomes have been observed. Heterotransplant of FB-1 cells into severe combined immunodeficient mice has resulted in rapidly growing tumors classified as anaplastic carcinomas, although 50% have shown areas with a trabecular pattern. FB-1 cells failed to express messenger RNA for thyroglobulin; TSH-receptor; thyroperoxidase, and placental angiogenic growth factor. Conversely, PAX8 and thyroid transcription factor 1, whose expression is thyroid specific, was kept in an FB-1 cell line at a level comparable with that observed in normal thyroid tissue. In addition, the present cell line expressed high levels of messenger RNA for high-mobility group proteins (Y) and -C. The in vitro study revealed that FB-1 cells are able to produce high levels of interleukin (IL)-8 and medium amount of IL-6, whereas no release of IL-1-, IL-1-ß, and IL-4 was observed. No modulation of cell proliferation and DNA synthesis in FB-1 cells has been observed after the addition of exogenous IL-6.

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