Journal of Clinical Endocrinology & Metabolism, Vol 81, 612-617, Copyright © 1996 by Endocrine Society

ARTICLES

Secretion of insulin-like growth factors and their binding proteins by human normal and hyperplastic prostatic cells in primary culture

C Boudon, G Rodier, E Lechevallier, N Mottet, B Barenton and C Sultan
INSERM U439, Hopital Lapeyronie, Montpellier, France.

Benign prostatic hyperplasia (BPH) is the most common benign proliferative disorder of unknown etiology found in men. Because insulin-like growth factors (IGFs) with their binding proteins (IGFBPs) are involved in the control of cellular proliferation, differentiation, and metabolism, we compared their secretion by prostatic epithelial and stromal cells in primary culture from the four different zones of normal prostate and from hyperplastic tissue to assess their contributions to the hyperplastic development. IGF-I could not be detected in the conditioned medium from either epithelial or stromal cells from normal and BPH tissues. IGF-II concentrations were the same in the conditioned medium from the epithelial cells of the different zones of the normal prostate and that of BPH cells. IGF-II concentrations secreted in stromal cell culture medium, however, were higher in the periurethral zone than in the peripheral and central zones. Moreover, in the periurethral zone, stromal cells secreted higher concentrations of IGF-II than did epithelial cells. Also, BPH stromal cells secreted more IGF-II than did BPH epithelial cells. IGFBP- 3, IGFBP-2, and IGFBP-4 were all secreted by both epithelial and stromal cells. In contrast, IGFBP-5 was only produced by stromal cells of the periurethral zone of the normal prostate and BPH tissue. IGFBP-3 was predominantly secreted by normal stromal cells of the transitional zone. We observed that BPH stromal cells presented the same pattern of IGF-II and IGFBP production as normal stromal cells of the periurethral zone. These data support the hypothesis that the periurethral zone is the main region of the prostate implicated in the development of BPH. They also suggest that the variability in both IGF-II secretion and the secreted forms of IGFBPs, depending on anatomical location within the organ, may be important for the autocrine regulation of normal and hyperplastic prostate growth.

This article has been cited by other articles:

				C. Chen, R. Freeman, L. F. Voigt, A. Fitzpatrick, S. R. Plymate, and N. S. Weiss Prostate Cancer Risk in Relation to Selected Genetic Polymorphisms in Insulin-like Growth Factor-I, Insulin-like Growth Factor Binding Protein-3, and Insulin-like Growth Factor-I Receptor Cancer Epidemiol. Biomarkers Prev., December 1, 2006; 15(12): 2461 - 2466. [Abstract] [Full Text] [PDF]

				R. O. Roberts, D. J. Jacobson, C. J. Girman, T. Rhodes, G. G. Klee, M. M. Lieber, and S. J. Jacobsen Insulin-like Growth Factor I, Insulin-like Growth Factor Binding Protein 3, and Urologic Measures of Benign Prostatic Hyperplasia Am. J. Epidemiol., May 1, 2003; 157(9): 784 - 791. [Abstract] [Full Text] [PDF]

				S. Monti, F. Di Silverio, R. Iraci, C. Martini, S. Lanzara, P. Falasca, M. Poggi, A. Stigliano, F. Sciarra, and V. Toscano Regional Variations of Insulin-Like Growth Factor I (IGF-I), IGF-II, and Receptor Type 1 in Benign Prostatic Hyperplasia Tissue and Their Correlation with Intraprostatic Androgens J. Clin. Endocrinol. Metab., April 1, 2001; 86(4): 1700 - 1706. [Abstract] [Full Text]

				S. M. Harman, E. J. Metter, M. R. Blackman, P. K. Landis, and H. B. Carter Serum Levels of Insulin-Like Growth Factor I (IGF-I), IGF-II, IGF-Binding Protein-3, and Prostate-Specific Antigen as Predictors of Clinical Prostate Cancer J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4258 - 4265. [Abstract] [Full Text]

				X. Zi, J. Zhang, R. Agarwal, and M. Pollak Silibinin Up-Regulates Insulin-like Growth Factor-Binding Protein 3 Expression and Inhibits Proliferation of Androgen-independent Prostate Cancer Cells Cancer Res., October 1, 2000; 60(20): 5617 - 5620. [Abstract] [Full Text]

				J. L. Martin and S. L. Pattison Insulin-Like Growth Factor Binding Protein-3 Is Regulated by Dihydrotestosterone and Stimulates Deoxyribonucleic Acid Synthesis and Cell Proliferation in LNCaP Prostate Carcinoma Cells Endocrinology, July 1, 2000; 141(7): 2401 - 2409. [Abstract] [Full Text] [PDF]

				H. Miyake, M. Pollak, and M. E. Gleave Castration-induced Up-Regulation of Insulin-like Growth Factor Binding Protein-5 Potentiates Insulin-like Growth Factor-I Activity and Accelerates Progression to Androgen Independence in Prostate Cancer Models Cancer Res., June 1, 2000; 60(11): 3058 - 3064. [Abstract] [Full Text] [PDF]

				V. Hwa, C. Tomasini-Sprenger, A. López Bermejo, R. G. Rosenfeld, and S. R. Plymate Characterization of Insulin-Like Growth Factor-Binding Protein-Related Protein-1 in Prostate Cells J. Clin. Endocrinol. Metab., December 1, 1998; 83(12): 4355 - 4362. [Abstract] [Full Text]

				S. E. Damon, L. Maddison, J. L. Ware, and S. R. Plymate Overexpression of an Inhibitory Insulin-Like Growth Factor Binding Protein (IGFBP), IGFBP-4, Delays Onset of Prostate Tumor Formation Endocrinology, August 1, 1998; 139(8): 3456 - 3464. [Abstract] [Full Text] [PDF]

				P. J. Russell, S. Bennett, and P. Stricker Growth factor involvement in progression of prostate cancer Clin. Chem., April 1, 1998; 44(4): 705 - 723. [Abstract] [Full Text] [PDF]

				A. A. Sinisi, A. Bellastella, D. Prezioso, M. R. Nicchio, T. Lotti, M. Salvatore, and D. Pasquali Different Expression Patterns of Somatostatin Receptor Subtypes in Cultured Epithelial Cells from Human Normal Prostate and Prostate Cancer J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2566 - 2569. [Abstract] [Full Text] [PDF]