Journal of Clinical Endocrinology & Metabolism, Vol 81, 4296-4300, Copyright © 1996 by Endocrine Society

ARTICLES

Nongenomic effects of aldosterone on phosphocreatine levels in human calf muscle during recovery from exercise

J Zange, K Muller, R Gerzer, K Sippel and M Wehling
Institute of Clinical Pharmacology, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Mannheim, Germany.

Nongenomic in vitro effects of aldosterone on the sodium-proton antiport and intracellular second messengers have been described in human mononuclear leukocytes, vascular smooth muscle cells, and endothelial cells. To test the potential physiological relevance of these effects, an in vivo 31P magnetic resonance spectroscopy study on the human calf at rest and during exercise was performed in 10 healthy volunteers receiving either 1 mg aldosterone or placebo iv in a double blind, randomized, cross-over trial. Spectra were analyzed for phosphocreatine, ATP, phosphomonoesters, inorganic intracellular phosphate, and intracellular pH. Resting values remained unchanged by aldosterone. After isometric contraction of the calf (50% body weight for 3 min), phosphocreatine recovered to significantly higher levels after application of aldosterone compared with placebo. Other parameters were not significantly changed by aldosterone. Effects appeared immediately after isometric contraction and, thus, occurred within 8 min of aldosterone administration. They are, therefore, likely to represent the first contemporary evidence of nongenomic in vivo effects of aldosterone in man. These findings also point to an involvement of aldosteron in the acute stress adaptation of cellular oxidative metabolism in human muscle physiology.

This article has been cited by other articles:

				R. M. LOSEL, E. FALKENSTEIN, M. FEURING, A. SCHULTZ, H.-C. TILLMANN, K. ROSSOL-HASEROTH, and M. WEHLING Nongenomic Steroid Action: Controversies, Questions, and Answers Physiol Rev, July 1, 2003; 83(3): 965 - 1016. [Abstract] [Full Text] [PDF]

				M. Estrada, J. L. Liberona, M. Miranda, and E. Jaimovich Aldosterone- and testosterone-mediated intracellular calcium response in skeletal muscle cell cultures Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E132 - E139. [Abstract] [Full Text] [PDF]

				E. Falkenstein, A. W. Norman, and M. Wehling Mannheim Classification of Nongenomically Initiated (Rapid) Steroid Action(s) J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 2072 - 2075. [Abstract] [Full Text]

				B. M. W. Schmidt, A. Montealegre, C. P. Janson, N. Martin, C. Stein-Kemmesies, A. Scherhag, M. Feuring, M. Christ, and M. Wehling Short Term Cardiovascular Effects of Aldosterone in Healthy Male Volunteers J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3528 - 3533. [Abstract] [Full Text] [PDF]

				M. Christ, A. Gunther, M. Heck, B. M. W. Schmidt, E. Falkenstein, and M. Wehling Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells Circulation, March 23, 1999; 99(11): 1485 - 1491. [Abstract] [Full Text] [PDF]

				H. Wackerhage, U. Hoffmann, D. Essfeld, D. Leyk, K. Mueller, and J. Zange Recovery of free ADP, Pi, and free energy of ATP hydrolysis in human skeletal muscle J Appl Physiol, December 1, 1998; 85(6): 2140 - 2145. [Abstract] [Full Text] [PDF]

				M. Christ and M. Wehling Cardiovascular steroid actions: swift swallows or sluggish snails? Cardiovasc Res, October 1, 1998; 40(1): 34 - 44. [Abstract] [Full Text] [PDF]