Journal of Clinical Endocrinology & Metabolism, Vol 80, 2378-2380, Copyright © 1995 by Endocrine Society

ARTICLES

Ad4BP in the human adrenal cortex and its disorders

H Sasano, S Shizawa, T Suzuki, K Takayama, T Fukaya, K Morohashi and H Nagura
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.

Ad4BP, a zinc finger DNA-binding protein, is a transcription factor that regulates the expression of the steroidogenic P450 genes. We performed immunoblotting and immunohistochemistry of Ad4BP in 34 human adrenal cortex specimens, which included adrenocortical adenomas and carcinomas. Immunoblotting revealed a single band of 53K, corresponding to the mol wt of Ad4BP. The immunohistochemical studies demonstrated that Ad4BP immunoreactivity was present exclusively in the nuclei of nearly all of the adrenocortical parenchymal cells in both the normal and the pathological human adrenal specimens. Ad4BP was immunostained with equal intensity and frequency among the different cell types. Ad4BP immunoreactivity was also observed in areas of marked degenerative changes, such as lipomyelomatous lesions, and in poorly differentiated carcinoma cells. These results suggest a close association of Ad4BP expression with the biological phenotype of adrenocortical parenchymal cells. Ad4BP therefore seems to play important roles in the induction and maintenance of the transcription of all steroidogenic P450 genes in human adrenocortical cells, even after malignant transformation.

This article has been cited by other articles:

				M. Doghman, T. Karpova, G. A. Rodrigues, M. Arhatte, J. De Moura, L. R. Cavalli, V. Virolle, P. Barbry, G. P. Zambetti, B. C. Figueiredo, et al. Increased Steroidogenic Factor-1 Dosage Triggers Adrenocortical Cell Proliferation and Cancer Mol. Endocrinol., December 1, 2007; 21(12): 2968 - 2987. [Abstract] [Full Text] [PDF]

				M. Mueller, A. Atanasov, I. Cima, N. Corazza, K. Schoonjans, and T. Brunner Differential Regulation of Glucocorticoid Synthesis in Murine Intestinal Epithelial Versus Adrenocortical Cell Lines Endocrinology, March 1, 2007; 148(3): 1445 - 1453. [Abstract] [Full Text] [PDF]

				M. H. Bassett, B. Mayhew, K. Rehman, P. C. White, F. Mantero, G. Arnaldi, P. M. Stewart, I. Bujalska, and W. E. Rainey Expression Profiles for Steroidogenic Enzymes in Adrenocortical Disease J. Clin. Endocrinol. Metab., September 1, 2005; 90(9): 5446 - 5455. [Abstract] [Full Text] [PDF]

				J. Seely, K. S. Amigh, T. Suzuki, B. Mayhew, H. Sasano, V. Giguere, J. Laganiere, B. R. Carr, and W. E. Rainey Transcriptional Regulation of Dehydroepiandrosterone Sulfotransferase (SULT2A1) by Estrogen-Related Receptor {alpha} Endocrinology, August 1, 2005; 146(8): 3605 - 3613. [Abstract] [Full Text] [PDF]

				I. Kurihara, H. Shibata, S. Kobayashi, N. Suda, Y. Ikeda, K. Yokota, A. Murai, I. Saito, W. E. Rainey, and T. Saruta Ubc9 and Protein Inhibitor of Activated STAT 1 Activate Chicken Ovalbumin Upstream Promoter-Transcription Factor I-mediated Human CYP11B2 Gene Transcription J. Biol. Chem., February 25, 2005; 280(8): 6721 - 6730. [Abstract] [Full Text] [PDF]

				K. J. Saner, T. Suzuki, H. Sasano, J. Pizzey, C. Ho, J. F. Strauss III, B. R. Carr, and W. E. Rainey Steroid Sulfotransferase 2A1 Gene Transcription Is Regulated by Steroidogenic Factor 1 and GATA-6 in the Human Adrenal Mol. Endocrinol., January 1, 2005; 19(1): 184 - 197. [Abstract] [Full Text] [PDF]

				A.-M. Lefrancois-Martinez, J. Bertherat, P. Val, C. Tournaire, N. Gallo-Payet, D. Hyndman, G. Veyssiere, X. Bertagna, C. Jean, and A. Martinez Decreased Expression of Cyclic Adenosine Monophosphate-Regulated Aldose Reductase (AKR1B1) Is Associated with Malignancy in Human Sporadic Adrenocortical Tumors J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 3010 - 3019. [Abstract] [Full Text] [PDF]

				Y. Sato, T. Suzuki, K. Hidaka, H. Sato, K. Ito, S. Ito, and H. Sasano Immunolocalization of Nuclear Transcription Factors, DAX-1 and COUP-TF II, in the Normal Human Ovary: Correlation with Adrenal 4 Binding Protein/ Steroidogenic Factor-1 Immunolocalization during the Menstrual Cycle J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3415 - 3420. [Abstract] [Full Text] [PDF]

				T. Suzuki, K. Takahashi, A. D. Darnel, T. Moriya, O. Murakami, T. Narasaka, J. Takeyama, and H. Sasano Chicken Ovalbumin Upstream Promoter Transcription Factor II in the Human Adrenal Cortex and Its Disorders J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2752 - 2757. [Abstract] [Full Text]

				E. M. Kaaijk, H. Sasano, T. Suzuki, J. F. Beek, and F. van der Veen Distribution of steroidogenic enzymes involved in androgen synthesis in polycystic ovaries: an immunohistochemical study Mol. Hum. Reprod., May 1, 2000; 6(5): 443 - 447. [Abstract] [Full Text] [PDF]

				C. Pilon, P. Mulatero, L. Barzon, F. Veglio, C. Garrone, M. Boscaro, N. Sonino, and F. Fallo Mutations in CYP11B1 Gene Converting 11{beta}-Hydroxylase into an Aldosterone-Producing Enzyme Are Not Present in Aldosterone-Producing Adenomas J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4228 - 4231. [Abstract] [Full Text]

				Y. Sadovsky and P. A. Crawford Developmental and Physiologic Roles of the Nuclear Receptor Steroidogenic Factor-I in the Reproductive System Reproductive Sciences, January 1, 1998; 5(1): 6 - 12. [Abstract] [PDF]

				K. Iino, H. Sasano, Y. Oki, N. Andoh, R.-W. Shin, T. Kitamoto, K. Totsune, K. Takahashi, H. Suzuki, H. Nagura, et al. Urocortin Expression in Human Pituitary Gland and Pituitary Adenoma J. Clin. Endocrinol. Metab., November 1, 1997; 82(11): 3842 - 3850. [Abstract] [Full Text] [PDF]

				K. L. Parker and B. P. Schimmer Steroidogenic Factor 1: A Key Determinant of Endocrine Development and Function Endocr. Rev., June 1, 1997; 18(3): 361 - 377. [Abstract] [Full Text]

				M. S. Ramayya, J. Zhou, T. Kino, J. H. Segars, C. A. Bondy, and G. P. Chrousos Steroidogenic Factor 1 Messenger Ribonucleic Acid Expression in Steroidogenic and Nonsteroidogenic Human Tissues: Northern Blot and in Situ Hybridization Studies J. Clin. Endocrinol. Metab., June 1, 1997; 82(6): 1799 - 1806. [Abstract] [Full Text] [PDF]