| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Clinical Endocrinology & Metabolism, Vol 80, 1876-1881, Copyright © 1995 by Endocrine Society
ARTICLES |
DD Brandon, TE Erickson, EJ Keenan, EY Strawn, MJ Novy, KA Burry, C Warner and GM Clinton
Department of Medicine, Oregon Health Sciences University, Portland 97201, USA.
Estrogen and progestin are believed to be important physiological regulators of uterine leiomyoma growth. We recently showed that progesterone receptor messenger ribonucleic acid (mRNA) and protein levels are increased in human uterine leiomyomas compared with those in myometrial biopsy tissue obtained from the same patient. To further characterize the molecular mechanisms underlying abnormal growth of uterine leiomyomas, we analyzed biopsy samples of tumor and adjacent normal myometrium for estrogen receptor (ER) gene expression. Northern analysis indicated that ER mRNA levels were increased 1.4-to 12.6-fold in leiomyoma compared with myometrium in all patients examined (n = 11), whereas beta-actin mRNA was not different between the two groups. The size of the primary ER mRNA transcript was 6.2 kilobases in both leiomyoma and myometrium, indicating no gross mutation of the ER gene. An ER protein of 66 kilodaltons was detected by Western blot analysis, and quantitative immunoassay of ER revealed 9448 +/- 1955 fmol/mg DNA in leiomyoma compared to 2827 +/- 979 fmol/mg DNA in myometrial tissue. Scatchard analysis of 17 beta-estradiol binding to cell-free extracts revealed enhanced binding capacity (per mg DNA) in leiomyoma tissue (n = 6) of about 6-fold, whereas ER binding affinity was not substantially different between the leiomyoma and adjacent myometrial tissues. We propose that increased expression of progesterone receptor in leiomyoma is most likely a consequence of overexpression of functional ER that results in increased end-organ sensitivity to estradiol.
This article has been cited by other articles:
 |
|
 |
|
  B. Van Voorhis A 41-Year-Old Woman With Menorrhagia, Anemia, and Fibroids: Review of Treatment of Uterine Fibroids JAMA, January 7, 2009; 301(1): 82 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-c. Kim, S. Ramachandran, S.-h. Baek, S.-H. Kwon, K.-Y. Kwon, S.-D. Cha, I. Bae, and C.-H. Cho Induction of Growth Inhibition and Apoptosis in Human Uterine Leiomyoma Cells by Isoliquiritigenin Reproductive Sciences, July 1, 2008; 15(6): 552 - 558. [Abstract] [PDF]
|
|
|
|
 |
|
 L.W. Jackson, M.D. Zullo, and J.M. Goldberg The association between heavy metals, endometriosis and uterine myomas among premenopausal women: National Health and Nutrition Examination Survey 1999-2002 Hum. Reprod., March 1, 2008; 23(3): 679 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Grifone, H. M. Haupt, V. Podolski, and J. J. Brooks Immunohistochemical Expression of Estrogen Receptors in Chondrosarcomas and Enchondromas International Journal of Surgical Pathology, January 1, 2008; 16(1): 31 - 37. [Abstract] [PDF]
|
|
|
|
|
|
N. Sen, N. C. Demirkan, N. Colakoglu, and S. E. Duzcan Are There Any Differences in the Expression of Hormonal Receptors and Proliferation Markers Between Uterine and Extrauterine Leiomyomas? International Journal of Surgical Pathology, January 1, 2008; 16(1): 43 - 47. [Abstract] [PDF]
|
|
|
|
 |
|
 M. H. Hassan, S. A. Salama, H. M. M. Arafa, F. M. A. Hamada, and A. Al-Hendy Adenovirus-Mediated Delivery of a Dominant-Negative Estrogen Receptor Gene in Uterine Leiomyoma Cells Abrogates Estrogen- and Progesterone-Regulated Gene Expression J. Clin. Endocrinol. Metab., October 1, 2007; 92(10): 3949 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.B. Moore, L. Castro, L. Yu, X. Zheng, X. Di, M.I. Sifre, G.E. Kissling, R.R. Newbold, C.D. Bortner, and D. Dixon Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration Hum. Reprod., October 1, 2007; 22(10): 2623 - 2631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hussein-Fikret, P. J. Fuller, and C. E. Gargett Expression of Steroid Receptor Coactivators in Cultured Cells From Paired Myometrial and Fibroid Tissues Reproductive Sciences, September 1, 2005; 12(6): 445 - 451. [Abstract] [PDF]
|
|
|
|
|
|
T. Yamada, S. Nakago, O. Kurachi, J. Wang, S. Takekida, H. Matsuo, and T. Maruo Progesterone down-regulates insulin-like growth factor-I expression in cultured human uterine leiomyoma cells Hum. Reprod., April 1, 2004; 19(4): 815 - 821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Mason, D. Grove-Strawser, B. S. Rubin, R. A. Nowak, and J. J. Castellot Jr. Estrogen Induces CCN5 Expression in the Rat Uterus in Vivo Endocrinology, February 1, 2004; 145(2): 976 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kanamori, K. Takakura, M. Mandai, M. Kariya, K. Fukuhara, T. Kusakari, C. Momma, H. Shime, H. Yagi, M. Konishi, et al. PEP-19 overexpression in human uterine leiomyoma Mol. Hum. Reprod., November 1, 2003; 9(11): 709 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chegini, J. Verala, X. Luo, J. Xu, and R. S. Williams Gene Expression Profile of Leiomyoma and Myometrium and the Effect of Gonadotropin Releasing Hormone Analogue Therapy Reproductive Sciences, April 1, 2003; 10(3): 161 - 171. [Abstract] [PDF]
|
|
|
|
|
|
F. Amant, E. Huys, A. Geurts-Moespot, B. G. Lindeque, I. Vergote, F. Sweep, and E. F. P. M. Schoenmakers Ethnic Variations in Uterine Leiomyoma Biology Are Not Caused By Differences in Myometrial Estrogen Receptor Alpha Levels Reproductive Sciences, February 1, 2003; 10(2): 105 - 109. [Abstract] [PDF]
|
|
|
|
|
|
N. Chegini, C. Ma, X.M. Tang, and R.S. Williams Effects of GnRH analogues, `add-back' steroid therapy, antiestrogen and antiprogestins on leiomyoma and myometrial smooth muscle cell growth and transforming growth factor-{beta} expression Mol. Hum. Reprod., December 1, 2002; 8(12): 1071 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Palomba, T. Russo, F. Orio Jr, L. Tauchmanova, E. Zupi, P. L. B. Panici, C. Nappi, A. Colao, G. Lombardi, and F. Zullo Effectiveness of combined GnRH analogue plus raloxifene administration in the treatment of uterine leiomyomas: a prospective, randomized, single-blind, placebo-controlled clinical trial Hum. Reprod., December 1, 2002; 17(12): 3213 - 3219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Palomba, F. Orio Jr., M. Morelli, T. Russo, M. Pellicano, E. Zupi, G. Lombardi, C. Nappi, P. L. B. Panici, and F. Zullo Raloxifene Administration in Premenopausal Women with Uterine Leiomyomas: A Pilot Study J. Clin. Endocrinol. Metab., August 1, 2002; 87(8): 3603 - 3608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Gargett, K. Bucak, M. Zaitseva, S. Chu, N. Taylor, P. J. Fuller, and P. A.W. Rogers Estrogen receptor-{alpha} and -{beta} expression in microvascular endothelial cells and smooth muscle cells of myometrium and leiomyoma Mol. Hum. Reprod., August 1, 2002; 8(8): 770 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukuhara, M. Kariya, M. Kita, H. Shime, T. Kanamori, C. Kosaka, A. Orii, J. Fujita, and S. Fujii Secreted Frizzled Related Protein 1 Is Overexpressed in Uterine Leiomyomas, Associated with a High Estrogenic Environment and Unrelated to Proliferative Activity J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1729 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Walker Role of Hormonal and Reproductive Factors in the Etiology and Treatment of Uterine Leiomyoma Recent Prog. Horm. Res., January 1, 2002; 57(1): 277 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Kovacs, A. Oszter, P. M. Gocze, J. L. Kornyei, and I. Szabo Comparative analysis of cyclin D1 and oestrogen receptor ({alpha} and {beta}) levels in human leiomyoma and adjacent myometrium Mol. Hum. Reprod., November 1, 2001; 7(11): 1085 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sampath, Y. Zhu, R. C. Winneker, and Z. Zhang Aberrant Expression of Cyr61, a Member of the CCN (CTGF/Cyr61/Cef10/NOVH) Family, and Dysregulation by 17{beta}-Estradiol and Basic Fibroblast Growth Factor in Human Uterine Leiomyomas J. Clin. Endocrinol. Metab., April 1, 2001; 86(4): 1707 - 1715. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sumitani, M. Shozu, T. Segawa, K. Murakami, H.-J. Yang, K. Shimada, and M. Inoue In Situ Estrogen Synthesized by Aromatase P450 in Uterine Leiomyoma Cells Promotes Cell Growth Probably Via an Autocrine/Intracrine Mechanism Endocrinology, October 1, 2000; 141(10): 3852 - 3861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Horiuchi, T. Nikaido, T. Yoshizawa, K. Itoh, Y. Kobayashi, T. Toki, I. Konishi, and S. Fujii HCG promotes proliferation of uterine leiomyomal cells more strongly than that of myometrial smooth muscle cells in vitro Mol. Hum. Reprod., June 1, 2000; 6(6): 523 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nisolle, S. Gillerot, F. Casanas-Roux, J. Squifflet, M. Berliere, and J. Donnez Immunohistochemical study of the proliferation index, oestrogen receptors and progesterone receptors A and B in leiomyomata and normal myometrium during the menstrual cycle and under gonadotrophin-releasing hormone agonist therapy Hum. Reprod., November 1, 1999; 14(11): 2844 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Benassayag, M. J. Leroy, V. Rigourd, B. Robert, J. C. Honore, T. M. Mignot, M. C. Vacher-Lavenu, C. Chapron, and F. Ferre Estrogen receptors (ERalpha /ERbeta ) in normal and pathological growth of the human myometrium: pregnancy and leiomyoma Am J Physiol Endocrinol Metab, June 1, 1999; 276(6): E1112 - E1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Englund, A. Blanck, I. Gustavsson, U. Lundkvist, P. Sjöblom, A. Norgren, and B. Lindblom Sex Steroid Receptors in Human Myometrium and Fibroids: Changes during the Menstrual Cycle and Gonadotropin-Releasing Hormone Treatment J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 4092 - 4096. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shimomura, H. Matsuo, T. Samoto, and T. Maruo Up-Regulation by Progesterone of Proliferating Cell Nuclear Antigen and Epidermal Growth Factor Expression in Human Uterine Leiomyoma J. Clin. Endocrinol. Metab., June 1, 1998; 83(6): 2192 - 2198. [Abstract] [Full Text]
|
|
|
|
|
|
E. Geimonen, E. Boylston, A. Royek, and J. Andersen Elevated Connexin-43 Expression in Term Human Myometrium Correlates with Elevated c-Jun Expression and Is Independent of Myometrial Estrogen Receptors J. Clin. Endocrinol. Metab., April 1, 1998; 83(4): 1177 - 1185. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lessl, M. Klotzbuecher, S. Schoen, A. Reles, K. Stockemann, and U. Fuhrmann Comparative Messenger Ribonucleic Acid Analysis of Immediate Early Genes and Sex Steroid Receptors in Human Leiomyoma and Healthy Myometrium J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2596 - 2600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. L. Gittoes, C. J. McCabe, J. Verhaeg, M. C. Sheppard, and J. A. Franklyn Thyroid Hormone and Estrogen Receptor Expression in Normal Pituitary and Nonfunctioning Tumors of the Anterior Pituitary J. Clin. Endocrinol. Metab., June 1, 1997; 82(6): 1960 - 1967. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
