| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Clinical Endocrinology & Metabolism, Vol 79, 554-560, Copyright © 1994 by Endocrine Society
ARTICLES |
EB Mawer, ME Hayes, SE Heys, M Davies, A White, MF Stewart and GN Smith
Manchester University Bone Disease Research Center, United Kingdom.
One of 16 human small cell lung cancer cell lines examined was shown to synthesize a metabolite resembling 1,25-dihydroxyvitamin D3 [1,25- (OH)2D3]. The NCI H82 line converted 25-hydroxyvitamin D3 (25OHD3) into a compound indistinguishable from 1,25-(OH)2D3 in 3 different high performance liquid chromatography systems. Electron impact mass spectra for the trimethylsilylethers of the metabolite and authentic 1,25- (OH)2D3 were indistinguishable. Binding to an anti-1,25-(OH)2D3 antibody was identical for the metabolite and authentic 1,25-(OH)2D3, whereas administration to rats in vivo caused equivalent stimulation of calcium transport measured in vitro in duodenal sacs. Activity of the H82 1 alpha-hydroxylase appears to be substrate dependent and is not stimulated by PTH, suggesting that it is similar to the enzyme expressed by activated macrophages and other cell types at extrarenal sites. Inhibition by ketoconazole indicates that, like the renal and extrarenal enzymes, the H82 enzyme is cytochrome P450 dependent. These data indicate that the H82 small cell lung cancer cell line constitutively expresses 25-hydroxyvitamin D3-1 alpha-hydroxylase and can synthesize 1,25-(OH)2D3.
This article has been cited by other articles:
 |
|
 |
|
  M. F Holick and T. C Chen Vitamin D deficiency: a worldwide problem with health consequences Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1080S - 1086S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Alpert and U. Shaikh The Effects of Vitamin D Deficiency and Insufficiency on the Endocrine and Paracrine Systems Biol Res Nurs, October 1, 2007; 9(2): 117 - 129. [Abstract] [PDF]
|
|
|
|
 |
|
 M. F. Holick The Vitamin D Epidemic and its Health Consequences J. Nutr., November 1, 2005; 135(11): 2739S - 2748S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F Holick Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease Am. J. Clinical Nutrition, December 1, 2004; 80(6): 1678S - 1688S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F Holick Vitamin D: importance in the prevention of cancers, type 1 diabetes, heart disease, and osteoporosis Am. J. Clinical Nutrition, March 1, 2004; 79(3): 362 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Huang, V. Papavasiliou, J. S. Rhim, R. L. Horst, and R. Kremer Targeted Disruption of the 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase Gene in ras-Transformed Keratinocytes Demonstrates That Locally Produced 1{alpha},25-Dihydroxyvitamin D3 Suppresses Growth and Induces Differentiation in an Autocrine Fashion Mol. Cancer Res., November 1, 2002; 1(1): 56 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Maas, K. Reus, B. Diesel, W.-I. Steudel, W. Feiden, U. Fischer, and E. Meese Amplification and Expression of Splice Variants of the Gene Encoding the P450 Cytochrome 25-Hydroxyvitamin D3 1,{{alpha}}-Hydroxylase (CYP 27B1) in Human Malignant Glioma Clin. Cancer Res., April 1, 2001; 7(4): 868 - 875. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Koren, I. Hadari-Naor, E. Zuck, C. Rotem, U. A. Liberman, and A. Ravid Vitamin D Is a Prooxidant in Breast Cancer Cells Cancer Res., February 1, 2001; 61(4): 1439 - 1444. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Jones, H. Ramshaw, A. Zhang, R. Cook, V. Byford, J. White, and M. Petkovich Expression and Activity of Vitamin D-Metabolizing Cytochrome P450s (CYP1{alpha} and CYP24) in Human Nonsmall Cell Lung Carcinomas Endocrinology, July 1, 1999; 140(7): 3303 - 3310. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Kitanaka, K.-i. Takeyama, A. Murayama, T. Sato, K. Okumura, M. Nogami, Y. Hasegawa, H. Niimi, J. Yanagisawa, T. Tanaka, et al. Inactivating Mutations in the 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase Gene in Patients with Pseudovitamin D-Deficiency Rickets N. Engl. J. Med., March 5, 1998; 338(10): 653 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takeyama, S. Kitanaka, T. Sato, M. Kobori, J. Yanagisawa, and S. Kato 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science, September 19, 1997; 277(5333): 1827 - 1830. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. B. Mawer, J. Walls, A. Howell, M. Davies, W. A. Ratcliffe, and N. J. Bundred Serum 1,25-Dihydroxyvitamin D May Be Related Inversely to Disease Activity in Breast Cancer Patients with Bone Metastases J. Clin. Endocrinol. Metab., January 1, 1997; 82(1): 118 - 122. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
