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Journal of Clinical Endocrinology & Metabolism, Vol 79, 466-469, Copyright © 1994 by Endocrine Society

ARTICLES

Progesterone synthesis by human luteal cells: modulation by estradiol

M Vega, L Devoto, O Castro and P Kohen
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.

To assess the role of estradiol (E2) upon progesterone (P4) synthesis, a well defined human midluteal cell system was used. A dose-dependent inhibition of P4 synthesis with and without hCG was induced by E2. In addition, E2 had a dose related cumulative effect on pregnenolone as compared with control experiments (2-fold, P < 0.05) as well as in hCG- stimulated conditions (3-fold, P < 0.005). On the other hand, the concentrations of 20 alpha-hydroxyprogesterone obtained in all experimental conditions were similar to control values, indicating that the catabolism of P4 was not modified. 3 beta-Hydroxysteroid dehydrogenase activity was significantly diminished (P < 0.05) in the presence of E2. Finally, the kinetic studies on P4 synthesis from pregnenolone showed a competitive type of inhibition with a K1 of 2.22 x 10(-6) mol/L. These data indicate an inhibition of 3 beta- hydroxysteroid dehydrogenase on human corpus luteum by E2.


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