help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burch, H. B.
Right arrow Articles by Burman, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burch, H. B.
Right arrow Articles by Burman, K. D.

Journal of Clinical Endocrinology & Metabolism, Vol 78, 1384-1391, Copyright © 1994 by Endocrine Society

ARTICLES

Thyrotropin receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves' ophthalmopathy

HB Burch, D Sellitti, SG Barnes, EV Nagy, RS Bahn and KD Burman
Endocrine-Metabolic Service, Kyle Metabolic Unit, Walter Reed Army Medical Center, Washington, D.C. 20307-5001.

Autoimmunity against the TSH receptor (hTSH-R) is known to be the proximate cause of thyroidal activation in Graves' disease, but has not been definitively linked to extrathyroidal manifestations of this disorder, such as ophthalmopathy and pretibial myxedema. In an effort to increase our knowledge concerning mechanisms responsible for Graves' ophthalmopathy, we used antiserum directed against a highly immunogenic portion of the hTSH-R (amino acids 352-367; P1) to assess the presence of this receptor or immunologically related protein in cultured human retroocular fibroblasts obtained from patients with Graves' ophthalmopathy. Immunoenzymatic and immunofluorescent studies revealed specific staining of both cytoplasmic and cell membrane-associated protein in discrete vesicles. To further evaluate the immunoreactive species present in these cells, immunoblotting experiments were performed using hTSH-R-specific antisera (anti-P1) and sera obtained from patients with Graves' disease. Several protein bands were identified using both anti-P1 and Graves' disease patient sera, including species at mol wt of 95, 71, and 18 kilodaltons, the possible significance of which is discussed. The results support the hypothesis that immunity against the hTSH-R or related proteins contributes to the ophthalmopathy of Graves' disease.


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
A. Schaffler, U. Muller-Ladner, J. Scholmerich, and C. Buchler
Role of Adipose Tissue as an Inflammatory Organ in Human Diseases
Endocr. Rev., August 1, 2006; 27(5): 449 - 467.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
M. Kazim, R. A. Goldberg, and T. J. Smith
Insights Into the Pathogenesis of Thyroid-Associated Orbitopathy: Evolving Rationale for Therapy
Arch Ophthalmol, March 1, 2002; 120(3): 380 - 386.
[Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. Bartalena, A. Pinchera, and C. Marcocci
Management of Graves' Ophthalmopathy: Reality and Perspectives
Endocr. Rev., April 1, 2000; 21(2): 168 - 199.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. S. Bahn, C. M. Dutton, N. Natt, W. Joba, C. Spitzweg, and A. E. Heufelder
Thyrotropin Receptor Expression in Graves' Orbital Adipose/Connective Tissues: Potential Autoantigen in Graves' Ophthalmopathy
J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 998 - 1002.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society