Journal of Clinical Endocrinology & Metabolism, Vol 78, 982-989, Copyright © 1994 by Endocrine Society

ARTICLES

Immunohistochemical localization, messenger ribonucleic acid abundance, and activity of 15-hydroxyprostaglandin dehydrogenase in placenta and fetal membranes during term and preterm labor

RK Sangha, JC Walton, CM Ensor, HH Tai and JR Challis
Department of Obstetrics and Gynecology, University of Western Ontario, Lawson Research Institute, St. Joseph's Health Center, London, Canada.

Type 1 15-hydroxyprostaglandin dehydrogenase (PGDH) is the main enzyme responsible for the metabolism of prostaglandin E2 (PGE2) and PGF2 alpha. To examine the possibility that a deficiency of PGDH might contribute to preterm labor, we measured localization of immunoreactive (IR-) PGDH, PGDH mRNA, and PGDH enzyme activity in chorio-decidua, placenta, and amnion in patients after term elective cesarean section (n = 9), after spontaneous vaginal term delivery (n = 10), and at idiopathic preterm labor (PTL) in the absence of infection (< 36 weeks gestation; n = 11). Localization of IR-PGDH was determined in additional specimens of membranes after PTL with infection (n = 13) and without (n = 37). IR-PGDH was localized in syncytiotrophoblast and intermediate trophoblasts in placenta and in the trophoblast layer of extraplacental chorion, but was absent from amnion in all patient groups. In chorion, the number of IR-positive trophoblasts was significantly reduced in the idiopathic PTL group compared to those in the other groups. The relative abundance of PGDH mRNA in the chorio- decidua, but not the placenta, from spontaneous labor and PTL was significantly less than that after cesarean section. PGDH mRNA in chorio-decidua from preterm patients correlated with PGDH enzyme activity. Undetectable or low IR-PGDH in chorionic trophoblasts was also associated with low enzyme activity. These results suggest that there exists a subset of patients that present in PTL because of reduced PGDH expression in chorionic trophoblasts. We suggest that this relative deficiency would allow PGs synthesized in the amnion or chorion to escape metabolism in the chorion and thereby contribute to the stimulus to idiopathic PTL.

This article has been cited by other articles:

				L. Gao, C. Lu, C. Xu, Y. Tao, B. Cong, and X. Ni Differential Regulation of Prostaglandin Production Mediated by Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 in Cultured Human Placental Trophoblasts Endocrinology, June 1, 2008; 149(6): 2866 - 2876. [Abstract] [Full Text] [PDF]

				V. Casciani, E. Marinoni, A. D. Bocking, M. Moscarini, R. Di Iorio, and J. R. G. Challis Opposite Effect of Phorbol Ester PMA on PTGS2 and PGDH mRNA Expression in Human Chorion Trophoblast Cells Reproductive Sciences, January 1, 2008; 15(1): 40 - 50. [Abstract] [PDF]

				J. D. Roizen, M. Asada, M. Tong, H.-H. Tai, and L. J. Muglia Preterm Birth without Progesterone Withdrawal in 15-Hydroxyprostaglandin Dehydrogenase Hypomorphic Mice Mol. Endocrinol., January 1, 2008; 22(1): 105 - 112. [Abstract] [Full Text] [PDF]

				R. M. Rizek, C. S. Watson, S. Keating, H.-H. Tai, J. R. G. Challis, and A. D. Bocking 15-Hydroxyprostaglandin Dehydrogenase Protein Expression in Human Fetal Membranes With and Without Subclinical Inflammation Reproductive Sciences, April 1, 2007; 14(3): 260 - 269. [Abstract] [PDF]

				K. Sayasith, N. Bouchard, M. Dore, and J. Sirois Cloning of equine prostaglandin dehydrogenase and its gonadotropin-dependent regulation in theca and mural granulosa cells of equine preovulatory follicles during the ovulatory process Reproduction, February 1, 2007; 133(2): 455 - 466. [Abstract] [Full Text] [PDF]

				D. M. Duffy, B. L. Dozier, and C. L. Seachord Prostaglandin Dehydrogenase and Prostaglandin Levels in Periovulatory Follicles: Implications for Control of Primate Ovulation by Prostaglandin E2 J. Clin. Endocrinol. Metab., February 1, 2005; 90(2): 1021 - 1027. [Abstract] [Full Text] [PDF]

				R. F. Johnson, C. M. Mitchell, V. Clifton, and T. Zakar Regulation of 15-Hydroxyprostaglandin Dehydrogenase (PGDH) Gene Activity, Messenger Ribonucleic Acid Processing, and Protein Abundance in the Human Chorion in Late Gestation and Labor J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5639 - 5648. [Abstract] [Full Text] [PDF]

				S. A. Tornblom, F. A. Patel, B. Bystrom, D. Giannoulias, A. Malmstrom, M. Sennstrom, S. J. Lye, J. R. G. Challis, and G. Ekman 15-Hydroxyprostaglandin Dehydrogenase and Cyclooxygenase 2 Messenger Ribonucleic Acid Expression and Immunohistochemical Localization in Human Cervical Tissue during Term and Preterm Labor J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 2909 - 2915. [Abstract] [Full Text] [PDF]

				T. J. Jerde, W. S. Mellon, S. M. Fischer, M. Liebert, D. E. Bjorling, and S. Y. Nakada Suppression of 15-Hydroxyprostaglandin Dehydrogenase Messenger RNA Concentration, Protein Expression, and Enzymatic Activity during Human Ureteral Obstruction J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 398 - 403. [Abstract] [Full Text]

				N. Mirazi, N. Alfaidy, R. Martin, and J. R. G. Challis Effects of Dexamethasone and Sulfasalazine on Prostaglandin E2 Output by Human Placental Cells In Vitro Reproductive Sciences, January 1, 2004; 11(1): 22 - 26. [Abstract] [PDF]

				F. A. Patel, J. W. Funder, and J. R. G. Challis Mechanism of Cortisol/Progesterone Antagonism in the Regulation of 15-Hydroxyprostaglandin Dehydrogenase Activity and Messenger Ribonucleic Acid Levels in Human Chorion and Placental Trophoblast Cells at Term J. Clin. Endocrinol. Metab., June 1, 2003; 88(6): 2922 - 2933. [Abstract] [Full Text] [PDF]

				K. J. McKeown and J. R. G. Challis Regulation of 15-Hydroxy Prostaglandin Dehydrogenase by Corticotrophin-Releasing Hormone through a Calcium-Dependent Pathway in Human Chorion Trophoblast Cells J. Clin. Endocrinol. Metab., April 1, 2003; 88(4): 1737 - 1741. [Abstract] [Full Text] [PDF]

				T. A. Sato, J. A. Keelan, and M. D. Mitchell Critical Paracrine Interactions Between TNF-{alpha} and IL-10 Regulate Lipopolysaccharide-Stimulated Human Choriodecidual Cytokine and Prostaglandin E2 Production J. Immunol., January 1, 2003; 170(1): 158 - 166. [Abstract] [Full Text] [PDF]

				D. M. Slater, S. Zervou, and S. Thornton Prostaglandins and Prostanoid Receptors in Human Pregnancy and Parturition Reproductive Sciences, May 1, 2002; 9(3): 118 - 124. [Abstract] [PDF]

				D. Giannoulias, F. A. Patel, A. C. Holloway, S. J. Lye, H. H. Tai, and J. R. G. Challis Differential Changes in 15-Hydroxyprostaglandin Dehydrogenase and Prostaglandin H Synthase (Types I and II) in Human Pregnant Myometrium J. Clin. Endocrinol. Metab., March 1, 2002; 87(3): 1345 - 1352. [Abstract] [Full Text] [PDF]

				F. A. Patel and J. R. G. Challis Cortisol/Progesterone Antagonism in Regulation of 15-Hydroxysteroid Dehydrogenase Activity and mRNA Levels in Human Chorion and Placental Trophoblast Cells at Term J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 700 - 708. [Abstract] [Full Text] [PDF]

				J. R. G. CHALLIS, S. J. LYE, W. GIBB, W. WHITTLE, F. PATEL, and N. ALFAIDY Understanding Preterm Labor Ann. N.Y. Acad. Sci., September 1, 2001; 943(1): 225 - 234. [Abstract] [Full Text] [PDF]

				W. X. Wu, X. H. Ma, T. Yoshizato, N. Shinozuka, and P. W. Nathanielsz Increase in Prostaglandin H Synthase 2, but Not Prostaglandin F2{alpha} Synthase mRNA in Intrauterine Tissues During Betamethasone-Induced Premature Labor and Spontaneous Term Labor in Sheep Reproductive Sciences, April 1, 2001; 8(2): 69 - 76. [Abstract] [PDF]

				W.L. Whittle, F.A. Patel, N. Alfaidy, A.C. Holloway, M. Fraser, S. Gyomorey, S.J. Lye, W. Gibb, and J.R.G. Challis Glucocorticoid Regulation of Human and Ovine Parturition: The Relationship Between Fetal Hypothalamic-Pituitary-Adrenal Axis Activation and Intrauterine Prostaglandin Production Biol Reprod, April 1, 2001; 64(4): 1019 - 1032. [Abstract] [Full Text]

				E. Schoof, M. Girstl, W. Frobenius, M. Kirschbaum, H. G. Dörr, W. Rascher, and J. Dötsch Decreased Gene Expression of 11{beta}-Hydroxysteroid Dehydrogenase Type 2 and 15-Hydroxyprostaglandin Dehydrogenase in Human Placenta of Patients with Preeclampsia J. Clin. Endocrinol. Metab., March 1, 2001; 86(3): 1313 - 1317. [Abstract] [Full Text]

				J. R.G. Challis, S. G. Matthews, W. Gibb, and S. J. Lye Endocrine and Paracrine Regulation of Birth at Term and Preterm Endocr. Rev., October 1, 2000; 21(5): 514 - 550. [Abstract] [Full Text]

				W. X. Wu, X. H. Ma, G. C. S. Smith, C. A. Mecenas, S. V. Koenen, and P. W. Nathanielsz Prostaglandin dehydrogenase mRNA in baboon intrauterine tissues in late gestation and spontaneous labor Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2000; 279(3): R1082 - R1090. [Abstract] [Full Text] [PDF]

				R. L. Goldenberg, J. C. Hauth, and W. W. Andrews Intrauterine Infection and Preterm Delivery N. Engl. J. Med., May 18, 2000; 342(20): 1500 - 1507. [Full Text] [PDF]

				K. J. Greenland, I. Jantke, S. Jenatschke, K. E. Bracken, C. Vinson, and B. Gellersen The Human NAD+-Dependent 15-Hydroxyprostaglandin Dehydrogenase Gene Promoter Is Controlled by Ets and Activating Protein-1 Transcription Factors and Progesterone Endocrinology, February 1, 2000; 141(2): 581 - 597. [Abstract] [Full Text] [PDF]

				Interleukin-10 Modifies the Effects of Interleukin-1{beta} and Tumor Necrosis Factor-{alpha} on the Activity and Expression of Prostaglandin H Synthase-2 and the NAD+-Dependent 15-Hydroxyprostaglandin Dehydrogenase in Cultured Term Human Villous Trophoblast and Chorion Trophoblast Cells J. Clin. Endocrinol. Metab., December 1, 1999; 84(12): 4645 - 4651. [Abstract] [Full Text]

				J. McLaren, D.J. Taylor, and S.C. Bell Increased incidence of apoptosis in non-labour-affected cytotrophoblast cells in term fetal membranes overlying the cervix Hum. Reprod., November 1, 1999; 14(11): 2895 - 2900. [Abstract] [Full Text] [PDF]

				F. A. Patel, K. Sun, and J. R. G. Challis Local Modulation by 11{beta}-Hydroxysteroid Dehydrogenase of Glucocorticoid Effects on the Activity of 15-Hydroxyprostaglandin Dehydrogenase in Human Chorion and Placental Trophoblast Cells J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 395 - 400. [Abstract] [Full Text]

				F. A. Patel, V. L. Clifton, K. Chwalisz, and J. R. G. Challis Steroid Regulation of Prostaglandin Dehydrogenase Activity and Expression in Human Term Placenta and Chorio-Decidua in Relation to Labor J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 291 - 299. [Abstract] [Full Text]

				V. L. Clifton, J. F. Telfer, A. J. Thompson, I. T. Cameron, T. G. Teoh, S. J. Lye, and J. R. G. Challis Corticotropin-Releasing Hormone and Proopiomelanocortin-Derived Peptides Are Present in Human Myometrium J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3716 - 3721. [Abstract] [Full Text]

				J. E. Mijovic, T. Zakar, T. K. Nairn, and D. M. Olson Prostaglandin Endoperoxide H Synthase (PGHS) Activity and PGHS-1 and -2 Messenger Ribonucleic Acid Abundance in Human Chorion Throughout Gestation and With Preterm Labor J. Clin. Endocrinol. Metab., April 1, 1998; 83(4): 1358 - 1367. [Abstract] [Full Text]

				C. A. van Meir, S. G. Matthews, M. J. N. C. Keirse, M. M. Ramirez, A. Bocking, and J. R. G. Challis 15-Hydroxyprostaglandin Dehydrogenase: Implications in Preterm Labor with and without Ascending Infection J. Clin. Endocrinol. Metab., March 1, 1997; 82(3): 969 - 976. [Abstract] [Full Text] [PDF]

				K. E. Bracken, W. Elger, I. Jantke, A. Nanninga, and B. Gellersen Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2{alpha} Secretion in Cultured Endometrial Cells Endocrinology, January 1, 1997; 138(1): 237 - 247. [Abstract] [Full Text] [PDF]