| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Clinical Endocrinology & Metabolism, Vol 78, 428-432, Copyright © 1994 by Endocrine Society
ARTICLES |
SE Bulun and ER Simpson
Cecil H. and Ida Green Center for Reproductive Biology Sciences, Department of Obstetrics-Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9051.
Circulating androstenedione is converted to estrone in adipose tissue, which is the principal site of estrogen biosynthesis in postmenopausal women. This reaction is catalyzed by a specific form of cytochrome P450 (P450arom; the product of the CYP19 gene). The fractional conversion of plasma androstenedione to estrone as well as the specific activity of aromatase in adipose stromal cells were previously shown to increase with advancing age. To determine whether this positive effect of aging on estrogen biosynthesis is due to an alteration in tissue levels of P450arom transcripts, we quantified P450arom mRNA levels in sc fat biopsy samples (n = 33) from buttocks, thighs, and abdomen of 11 women who ranged in age from 23-61 yr. Competitive polymerase chain reaction linked to reverse transcription was used to quantify P450arom transcripts in total RNA that was isolated from sc fat obtained by needle aspiration. In each sample, primer extension and coamplification of a rat P450arom cRNA as an internal standard were used to control possible differences in amplification efficiencies between samples. The results demonstrate that with advancing age in women, there is a progressive and statistically significant increase in adipose tissue P450arom transcript levels (normalized to total RNA content) in buttocks, thighs, and abdomen (correlation coefficients: r = 0.704, 0.854, and 0.933, respectively). The levels of transcripts observed in the older subjects reach 2- to 4-fold greater than those observed in the young women. Adipose tissue P450arom transcript levels were highest in the buttocks, followed by the thighs, and lowest in the abdomen. This increase in P450arom transcript levels is likely to be a major factor contributing to the increased extragonadal estrogen biosynthesis in elderly women.
This article has been cited by other articles:
 |
|
 |
|
  M. Tanaka, R. Koga, H. Tsuda, K. Imai, S. Abe, T. Masuda, M. Iwamoto, E. Nakazono, T. Kamohara, N. Kinukawa, et al. Subcutaneous Fat Accumulation Shows a Beneficial Correlation with Serum Cholesterol in Postmenopausal Japanese Women Experimental Biology and Medicine, September 1, 2007; 232(8): 1064 - 1070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Panjari and S. R. Davis DHEA therapy for women: effect on sexual function and wellbeing Hum. Reprod. Update, May 1, 2007; 13(3): 239 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. I. Perez, A. Jurisicova, L. Wise, T. Lipina, M. Kanisek, A. Bechard, Y. Takai, P. Hunt, J. Roder, M. Grynpas, et al. Absence of the proapoptotic Bax protein extends fertility and alleviates age-related health complications in female mice PNAS, March 20, 2007; 104(12): 5229 - 5234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Olson, E. V. Bandera, and I. Orlow Variants in Estrogen Biosynthesis Genes, Sex Steroid Hormone Levels, and Endometrial Cancer: A HuGE Review Am. J. Epidemiol., February 1, 2007; 165(3): 235 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Wierman, R. Basson, S. R. Davis, S. Khosla, K. K. Miller, W. Rosner, and N. Santoro Androgen Therapy in Women: An Endocrine Society Clinical Practice Guideline J. Clin. Endocrinol. Metab., October 1, 2006; 91(10): 3697 - 3710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Honma, K. Takubo, M. Sawabe, T. Arai, F. Akiyama, G. Sakamoto, T. Utsumi, N. Yoshimura, and N. Harada Estrogen-Metabolizing Enzymes in Breast Cancers from Women over the Age of 80 Years J. Clin. Endocrinol. Metab., February 1, 2006; 91(2): 607 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Attar and S.E. Bulun Aromatase and other steroidogenic genes in endometriosis: translational aspects Hum. Reprod. Update, January 1, 2006; 12(1): 49 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bulun, Z. Lin, G. Imir, S. Amin, M. Demura, B. Yilmaz, R. Martin, H. Utsunomiya, S. Thung, B. Gurates, et al. Regulation of Aromatase Expression in Estrogen-Responsive Breast and Uterine Disease: From Bench to Treatment Pharmacol. Rev., September 1, 2005; 57(3): 359 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. de Ronde, A. Hofman, H. A P Pols, and F. H de Jong A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women Eur. J. Endocrinol., February 1, 2005; 152(2): 261 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Fan, T. Yanase, H. Morinaga, Y.-M. Mu, M. Nomura, T. Okabe, K. Goto, N. Harada, and H. Nawata Activation of Peroxisome Proliferator-Activated Receptor-{gamma} and Retinoid X Receptor Inhibits Aromatase Transcription via Nuclear Factor-{kappa}B Endocrinology, January 1, 2005; 146(1): 85 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bjornerem, B. Straume, M. Midtby, V. Fonnebo, J. Sundsfjord, J. Svartberg, G. Acharya, P. Oian, and G. K. R. Berntsen Endogenous Sex Hormones in Relation to Age, Sex, Lifestyle Factors, and Chronic Diseases in a General Population: The Tromso Study J. Clin. Endocrinol. Metab., December 1, 2004; 89(12): 6039 - 6047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Somboonporn and S. R. Davis Testosterone Effects on the Breast: Implications for Testosterone Therapy for Women Endocr. Rev., June 1, 2004; 25(3): 374 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shozu, S. Sebastian, K. Takayama, W.-T. Hsu, R. A. Schultz, K. Neely, M. Bryant, and S. E. Bulun Estrogen Excess Associated with Novel Gain-of-Function Mutations Affecting the Aromatase Gene N. Engl. J. Med., May 8, 2003; 348(19): 1855 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Rubin, J. H. Duong, C. D. Clyne, C. J. Speed, Y. Murata, C. Gong, and E. R. Simpson Ligands for the Peroxisomal Proliferator-Activated Receptor {gamma} and the Retinoid X Receptor Inhibit Aromatase Cytochrome P450 (CYP19) Expression Mediated by Promoter II in Human Breast Adipose Endocrinology, August 1, 2002; 143(8): 2863 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. McTernan, L. A. Anderson, A. J. Anwar, M. C. Eggo, J. Crocker, A. H. Barnett, P. M. Stewart, and S. Kumar Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences J. Clin. Endocrinol. Metab., March 1, 2002; 87(3): 1327 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Wajchenberg Subcutaneous and Visceral Adipose Tissue: Their Relation to the Metabolic Syndrome Endocr. Rev., December 1, 2000; 21(6): 697 - 738. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. L. Rubin, Y. Zhao, A. M. Kalus, and E. R. Simpson Peroxisome Proliferator-activated Receptor {{gamma}} Ligands Inhibit Estrogen Biosynthesis in Human Breast Adipose Tissue: Possible Implications for Breast Cancer Therapy Cancer Res., March 1, 2000; 60(6): 1604 - 1608. [Abstract] [Full Text]
|
|
|
|
|
|
G. A. Laughlin, E. Barrett-Connor, D. Kritz-Silverstein, and D. von Mühlen Hysterectomy, Oophorectomy, and Endogenous Sex Hormone Levels in Older Women: The Rancho Bernardo Study J. Clin. Endocrinol. Metab., February 1, 2000; 85(2): 645 - 651. [Abstract] [Full Text]
|
|
|
|
|
|
F. Machinal, M.-N. Dieudonne, M.-C. Leneveu, R. Pecquery, and Y. Giudicelli In Vivo and in Vitro ob Gene Expression and Leptin Secretion in Rat Adipocytes: Evidence for a Regional Specific Regulation by Sex Steroid Hormones Endocrinology, April 1, 1999; 140(4): 1567 - 1574. [Abstract] [Full Text]
|
|
|
|
|
|
L. Dalla Valle, A. Ramina, S. Vianello, A. Fassina, P. Belvedere, and L. Colombo Potential for Estrogen Synthesis and Action in Human Normal and Neoplastic Thyroid Tissues J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3702 - 3709. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Reed and A. Purohit Breast Cancer and the Role of Cytokines in Regulating Estrogen Synthesis: An Emerging Hypothesis Endocr. Rev., October 1, 1997; 18(5): 701 - 715. [Abstract] [Full Text]
|
|
|
|
|
|
V. R. Agarwal, C. I. Ashanullah, E. R. Simpson, and S. E. Bulun Alternatively Spliced Transcripts of the Aromatase Cytochrome P450 (CYP19) Gene in Adipose Tissue of Women J. Clin. Endocrinol. Metab., January 1, 1997; 82(1): 70 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, J. E. Nichols, S. E. Bulun, C. R. Mendelson, and E. R. Simpson Aromatase P450 Gene Expression in Human Adipose Tissue J. Biol. Chem., July 7, 1995; 270(27): 16449 - 16457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Nichols, S. E. Bulun, and E. R. Simpson Effects of Conditioned Medium from Different Cultured Cell Types on Aromatase Expression in Adipose Stromal Cells Reproductive Sciences, January 1, 1995; 2(1): 45 - 50. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
