The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase
NA Hey, RA Graham, MW Seif and JD Aplin
Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
After ovulation, progesterone stimulates a temporally regulated secretory
transformation in human endometrial epithelium. Using a combination of
immunohistochemistry, and Western and Northern blotting, we demonstrate
that 1) the polymorphic epithelial mucin MUC1 is secreted by human
endometrial epithelium; 2) low levels of both mRNA and core protein are
present in the preovulatory phase of the menstrual cycle; 3) mRNA levels
increase several-fold after ovulation, consistent with transcriptional
regulation by progesterone; 4) there is an increase in translation product
in postovulatory endometrium; and 5) the tandem repeat domain of the MUC-1
polypeptide is glycosylated in endometrium.
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