Journal of Clinical Endocrinology & Metabolism, Vol 75, 1545-1549, Copyright © 1992 by Endocrine Society

ARTICLES

Presence of messenger ribonucleic acid for endothelin-1, endothelin-2, and endothelin-3 in human endometrium and a change in the ratio of ETA and ETB receptor subtype across the menstrual cycle

G O'Reilly, DS Charnock-Jones, AP Davenport, IT Cameron and SK Smith
Department of Obstetrics and Gynaecology, University of Cambridge Clinical School, United Kingdom.

The aim of the present study was to determine whether mRNA for the three endothelin peptides (endothelin-1, endothelin-2, and endothelin- 3) and the two known receptor subtypes (ETA and ETB) was present in human endometrium at different stages of the menstrual cycle (menstrual, early and mid-proliferative, and early, mid-, and late secretory). Endometrium was obtained from women undergoing surgery for benign disease, and total RNA was extracted using a guanidinium isothiocyanate method. mRNA for endothelin peptide and receptor was detected using the reverse transcriptase-polymerase chain reaction with nested oligonucleotide primers. mRNA for endothelin-1, endothelin-2, and endothelin-3 was demonstrated throughout the menstrual cycle, and three splice variants of mRNA encoding endothelin-3 were found in all samples. The ratio of ETA to ETB receptor mRNA was found to change throughout the menstrual cycle. In the proliferative phase, amplified cDNA product was almost exclusively confined to the ETA receptor, whereas an increase in the amplified product of the ETB receptor cDNA was seen in the secretory and menstrual phases. These studies show that mRNA for endothelin-1, endothelin-2, and endothelin-3 is present in human endometrium at all stages of the menstrual cycle and suggest that different physiological actions of the endothelin peptides may be mediated through changes in the ratio of the ETA and ETB receptor subtypes.

This article has been cited by other articles:

				A. Iwase, H. Ando, T. Nagasaka, D. Shibata, T. Harata, Y. Shimomura, M. Goto, and F. Kikkawa Neutral Endopeptidase Expressed by Decidualized Stromal Cells Suppresses Akt Phosphorylation and Deoxyribonucleic Acid Synthesis Induced by Endothelin-1 in Human Endometrium Endocrinology, November 1, 2006; 147(11): 5153 - 5159. [Abstract] [Full Text] [PDF]

				H. N. Jabbour, R. W. Kelly, H. M. Fraser, and H. O. D. Critchley Endocrine Regulation of Menstruation Endocr. Rev., February 1, 2006; 27(1): 17 - 46. [Abstract] [Full Text] [PDF]

				M. Edlund, E. Andersson, and G. Fried Progesterone withdrawal causes endothelin release from cultured human uterine microvascular endothelial cells Hum. Reprod., June 1, 2004; 19(6): 1272 - 1280. [Abstract] [Full Text] [PDF]

				V. PINGAULT, N. BONDURAND, N. LEMORT, M. SANCANDI, I. CECCHERINI, J.-P. HUGOT, P.-S. JOUK, and M. GOOSSENS A heterozygous endothelin 3 mutation in Waardenburg-Hirschsprung disease: is there a dosage effect of EDN3/EDNRB gene mutations on neurocristopathy phenotypes? J. Med. Genet., March 1, 2001; 38(3): 205 - 209. [Full Text]

				F. Miceli, F. Minici, M. G. Pardo, P. Navarra, C. Proto, S. Mancuso, A. Lanzone, and R. Apa Endothelins Enhance Prostaglandin (PGE2 and PGF2{{alpha}}) Biosynthesis and Release by Human Luteal Cells: Evidence of a New Paracrine/Autocrine Regulation of Luteal Function J. Clin. Endocrinol. Metab., February 1, 2001; 86(2): 811 - 817. [Abstract] [Full Text]

				T. Uchide, H. Masuda, Y.-S. Lee, Y. Makiyama, Y. Mitsui, and K. Saida Fluctuating Gene Expression and Localized Cellular Distribution of Vasoactive Intestinal Contractor (VIC) in Mouse Uterus J. Histochem. Cytochem., May 1, 2000; 48(5): 699 - 708. [Abstract] [Full Text]

				J. Andersen and R. L. Barbieri Abnormal Gene Expression in Uterine Leiomyomas Reproductive Sciences, September 1, 1995; 2(5): 663 - 672. [Abstract] [PDF]