help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bhaumick, B.
Right arrow Articles by Bala, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bhaumick, B.
Right arrow Articles by Bala, R. M.

Journal of Clinical Endocrinology & Metabolism, Vol 74, 1005-1011, Copyright © 1992 by Endocrine Society

ARTICLES

Potentiation of epidermal growth factor-induced differentiation of cultured human placental cells by insulin-like growth factor-I

B Bhaumick, D George and RM Bala
Department of Medicine, University of Saskatchewan, Sashatoon, Canada.

Insulin-like growth factor-I (IGF-I) potentiates epidermal growth factor (EGF)-stimulated placental lactogen (hPL) secretion by cultured placental cells. In this study we have examined the effects of EGF and IGF-I, alone or in combination, on the differentiation of placental cells in culture and correlated this differentiation with hPL secretion. Addition of EGF (10 ng/mL) or IGF-I (100 ng/mL) alone in serum-free medium was associated with enlargement of the BhCG-positive (differentiated cytotrophoblast marker) mononucleated trophoblast cells. Concomitant addition of IGF-I and EGF resulted in more marked enlargement of the BhCG-positive cells, aggregation of the enlarged cells, and early syncytiotrophoblast formation. Measurement of hPL in the medium revealed that the stimulatory effect (P less than 0.05) of EGF on hPL secretion by total placental cells was proportional to the stimulatory effect of EGF on cell differentiation, as revealed by the obliteration of its stimulatory effect on hPL by BhCG-positive cells. The augmented stimulatory effect of EGF on hPL secretion by all cells in the presence of IGF-I was decreased (P less than 0.05) when expressed per number of human chorionic gonadotropin (BhCG)-positive cells. The dose-response curves of hPL stimulation by IGF-I in the presence of EGF revealed that the decreased effect of IGF-I on hPL secretion by BhCG-positive-staining cells was due to the difference in magnitude (approximately 1.5 times) of stimulation of BhCG staining of and hPL secretion by the cells. These results extend our previous suggestion that IGF-I in placenta, in addition to its effect on hPL secretion, affects cell differentiation; however, the two effects may not be interdependent.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1992 by The Endocrine Society