This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Articles by TANBO, T. Articles by HANSSON, V. Search for Related Content PubMed PubMed Citation Articles by TANBO, T. Articles by HANSSON, V.

Hormone-Responsive Adenylyl Cyclase in the Human Fallopian Tube^*

Departments of Obstetrics and Gynecology, National Hospital Oslo, Norway
Department of Medicine B, National Hospital Oslo, Norway
The Institute of Pathology, University of Oslo Oslo, Norway
The Institute of Medical Biochemistry, University of Oslo Oslo, Norway

Address requests for reprints to: Dr. Tom Tanbo, Department of Obstetrics and Gynecology, National Hospital, Oslo, Norway.

Hormone-responsive adenylyl cyclase (AC) activity in biopsies from normal human Fallopian tubes was studied. Enzyme activity with a K_m of 0.15 mmol/L and a maximum velocity of 13.8 pmol/mg·min in the basal condition was demonstrated. The addition of prostaglandin E₁ (PCE₁), PGE₂, PGF₂, vasoactive intestinal polypeptide, isoproterenol, and terbutaline increased enzyme activity, with no change in the K_m. Maximum stimulation of AC activity was obtained with PGE₁, resulting in a 2- to 8-fold increase in AC activity.

The response of AC to PGE₁ revealed a possible topographical variation, with lowest responses to PGE₁ in the isthmus. No such segmental variation in AC activity and response was seen after stimulation with PGF₂, vasoactive intestinal polypeptide, or isoproterenol.

^* This work was supported by grants from the Norwegian Cancer Society, Torsted’s Foundation, the Nordic Insulin Foundation, Dr. Alexander Malthe’s Foundation, and the Norwegian Research Council for Science and the Humanities.

Received December 27, 1989.