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Demonstration of the Existence of the Alternatively Spliced Form of Thyroid Peroxidase in Normal Thyroid^*

Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucleaire (R.E., G.V., M.L.) and Service de Génétique Medicale (G.V.), Uniuersité Libre de Bruxelles Brussels, Belgium

Address all correspondence and requests for reprints to: Dr. M. Ludgate, Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucleaire, Université Libre de Bruxelles, Hopital Erasme, B-1070 Brussels, Belgium.

We have investigated whether the alternatively spliced form of thyroid peroxidase (TPO), which has been shown clearly to occur in Graves’ thyroid, is present in normal thyroid. We have performed a polymerase chain reaction on cDNAs prepared from normal thyroid mRNAs using primers 100 bases up-stream and down-stream from the 171 nucleotides that have been shown to be spliced out. Size analysis of the polymerase chain reaction products by agarose gel electrophoresis revealed bands at 0.2 and 0.37 kilobases (kb), sizes predicted to be obtained when the two forms of TPO cDNA are present. This was confirmed by hybridization with a ³²P-labeled probe corresponding to the 20 nucleotides of the junction site. After stringent washing, the 0.2-kb band gave a clear positive signal, which was stronger than that of the 0.37-kb band despite the latter cDNA being more abundant. This demonstrates clearly that the alternatively spliced form of TPO exists in normal thyroid. Its significance is discussed.

^* This work was supported by grants from Ministero della Pubblica Istruzione Italia, the Wellcome Foundation, and NIH Sciences de la Vie.

Received July 16, 1990.

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