| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Clinical Endocrinology & Metabolism, Vol 72, 387-391, Copyright © 1991 by Endocrine Society
ARTICLES |
T Jan, MA Shaw and G Baumann
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
GH-binding proteins (GH-BPs) in human blood theoretically may interfere with measurements of immunoreactive GH by forming complexes with GH and competing with antibody reagents for ligand. Indeed, results of serum GH obtained by immunoassays are known to differ markedly depending on the assay employed. To assess the potential role of circulating GH-BPs in this phenomenon, we systematically examined their effect on GH measurement in four RIAs and two immunoradiometric assays. In all except one RIA, the effect of the BPs on tracer binding to antibody was mildly inhibitory. In both immunoradiometric assays, BPs increased the nonspecific association of the tracer with the solid phase reagent. However, these effects were minor at the BP concentrations realistically encountered in practice. Furthermore, the impact of BPs on GH standard curves and final results was negligible because BP effects fall into the category of nonspecific or zero dose counts, which are subtracted during data reduction. We conclude that GH-BPs are only a minor disturbance in GH immunoassays, which is completely compensated for by conventional assay design. Disparities among results yielded by different assays are probably not due to BP interference.
This article has been cited by other articles:
![]() |
B. C. Nindl, W. C. Hymer, D. R. Deaver, and W. J. Kraemer Growth hormone pulsatility profile characteristics following acute heavy resistance exercise J Appl Physiol, July 1, 2001; 91(1): 163 - 172. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Andersen, P. H. Petersen, O. Blaabjerg, J. Hangaard, and C. Hagen Evaluation of growth hormone assays using ratio plots Clin. Chem., May 1, 1998; 44(5): 1032 - 1038. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Tzanela, C. Wagner, and G. Shaffer Tannenbaum Recombinant Human Growth Hormone-Binding Protein Fails to Enhance the in Vivo Bioactivity of Human Growth Hormone in Normal Rats Endocrinology, December 1, 1997; 138(12): 5316 - 5324. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Jansson, C. Boguszewski, S. Rosberg, L. Carlsson, and K. Albertsson-Wikland Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein Clin. Chem., June 1, 1997; 43(6): 950 - 956. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. L. Stock, M. R. Warth, B. T. Teh, J. A. Coderre, J. H. Overdorf, G. Baumann, R. L. Hintz, M. L. Hartman, B. R. Seizinger, C. Larsson, et al. A Kindred with a Variant of Multiple Endocrine Neoplasia Type 1 Demonstrating Frequent Expression of Pituitary Tumors but Not Linked to the Multiple Endocrine Neoplasia Type 1 Locus at Chromosome Region 11q13 J. Clin. Endocrinol. Metab., February 1, 1997; 82(2): 486 - 492. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |