Journal of Clinical Endocrinology & Metabolism, Vol 71, 1363-1367, Copyright © 1990 by Endocrine Society

ARTICLES

Human follicular fluids contain tissue macrophages

JA Loukides, RA Loy, R Edwards, J Honig, I Visintin and ML Polan
Department of Obstetrics and Gynecology, University of Maryland, Baltimore 21201.

Tissue cells obtained from the follicular fluids of women undergoing in vitro fertilization and embryo transfer were found to contain interleukin-1 beta mRNA by Northern analysis. Since macrophages are known to produce interleukin-1 beta, we examined the follicular fluids of 20 women undergoing in vitro fertilization as well as tissue sections of normal human ovary for the presence of macrophages and monocytes. Although granulosa-luteal cells predominate in follicular fluid, we found that resident macrophages and monocytes comprise 5-15% of human follicular tissue cells. In addition, we observed that macrophages are present in the human ovarian follicle as well as in the corpus luteum.

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