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Normal and Adenomatous Human Pituitaries Secrete Thyrotropin-Releasing Hormone In vitro: Modulation by Dopamine, Haloperidol, and Somatostatin

INSERM U.223, Faculté de Médecine Pitié-Salpétrière (M.L.D., P.L., CM., A.M.B., F.P.) 75634 Paris Cedex 13; France
Groupe de Neuroendocrinologie Cellulaire, Collège de France (A.B.) 75005 Paris; France
Fondation de Recherche en Hormonologie (M.C.F.) 94268 Fresnes Cedex, France

Address all correspondence and requests for reprints to: M. Le Dafniet, INSERM U.223, Faculté de Médecine Pitié-Salpétrière, 105 boulevard de l’Hôpital, 75634 Paris Cedex 13, France.

TRH is present in human normal pituitaries and in pituitary adenomas. In this study we demonstrated that the same tissues can release TRH in vitro. Fragments from seven normal pituitaries (10–15 mg/syringe) and dispersed cells from eight prolactinomas, four GH-secreting and two nonsecreting adenomas (1–3 x 10⁶ cells/syringe) were perifused using a Krebs-Ringer culture medium. After 1 h of equilibration the perifusion medium was collected every 2 min (1 mL/fraction) for 3 h. TRH, PRL, and GH were measured by RIA under basal conditions and in the presence of 10^–10 to 10^–6 mol/L dopamine (DA), alone or concomitant with haloperidol, or in the presence of 11^–10 or 10^–6 mol/L somatostatin. Both normal pituitary fragments and pituitary adenomatous cells (from all types of adenomas studied) spontaneously released TRH in vitro. TRH was detected in the perifusion medium either immediately after the end of the equilibration period or 30–60 min later. The molecular identity of TRH was assessed by high pressure liquid chromatography. There was no difference in the profile and the rate of TRH secretion between normal and tumoral tissues, and no correlation was found between the level of TRH release and that of PRL or GH secretion. DA stimulated TRH release from normal pituitaries and from PRL- and GH-secreting adenomas at doses as low as 10^–10 mol/L. A concomitant decrease in PRL and GH release was observed from adenomatous cells and in one case of normal tissue. Haloperidol (10^–7 mol/L) antagonized the effect of 10^–8 mol/L DA on both TRH and PRL secretion in normal pituitary and in prolactinomas. DA had no effect on TRH release from two nonsecreting tumors. The amounts of TRH released during 1 h of perifusion were 60–1640 pg/2 mg wet wt tissue in normal pituitaries and 54–2174 pg/10⁶ cells in adenomas; these values were very high compared to those precedently reported within the tissues.

These results indicate that pituitary cells can release TRH in vitro and suggest that TRH might be synthesized in situ. We suggest that TRH could act on pituitary hormone secretion and/or cell proliferation via a paracrine and/or an autocrine mechanism.

Received October 17, 1989.

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