help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Journal of Clinical Endocrinology & Metabolism Vol. 71, No. 2 474-479
doi:10.1210/jcem-71-2-474
Copyright © 1990 by the Endocrine Society.
This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by AMIT, T.
Right arrow Articles by HOCHBERG, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by AMIT, T.
Right arrow Articles by HOCHBERG, Z.

A New and Convenient Assay of Growth Hormone Binding Protein Activity in Human Serum

TAMAR AMIT*, RONNIE J. BARKEY, MOUSSA B. H. YOUDIM and ZEEV HOCHBERG

Rappaport Family Institute for Research in the Medical Sciences and the Department of Pharmacology Faculty of Medicine Technion, Haifa, Israel

Address all correspondence and requests for reprints to: Dr. Zeev Hochberg, Rappaport Family Research Institute, Technion, P.O.B. 9697, Haifa 31096, Israel.

The recent discovery of a GH-binding protein (GH-BP) in human serum has important implications for the distribution, metabolism, and physiological activity of human GH (hGH). In this report we describe an accurate, simple, and rapid assay for measuring GH-BP in human serum, based onthe dextran-coated charcoal technique for separating bound from free hGH. The present method reduces the degree of dissociation of the GH-BP complex during the separation procedure, thus increasing accuracy, allows routine use of small working volumes of sera, and can be practically used for running large scale assays. Binding of [125I]hGH to human serum GH-BP, identified and characterized by this procedure, was time, temperature, and dose dependent; the binding was highly specific for hGH and with a high affinity (Ka, 1.08 ± 0.19 x 109 L/mol). Preliminary results, expressed as percent specifically bound [125I]hGH corrected for endogenous hGH, indicate that the mean GH-BP activity in 50 µL sera from normal adult men and women was 11.32 ± 0.45%. Significantly lower values were obtained in sera of infants, 3 days after birth (1.65 ± 0.15%), and in short-statured children with normal GH secretion (7.38 ± 0.22%). In patients with liver cirrhosis GH-BP levels decreased to 5.93 ± 1.14%.

The availability of a simple and convenient procedure for large scale determination of GH-BP coupled with the suggestion that this protein can reflect the GH receptor provides us with an indirect means for assessing changes in GH receptor activity in various physiological and pathological conditions.

* Supported by a postdoctoral fellowship from the Wolf Foundation, Israel.

Received July 20, 1989.




This article has been cited by other articles:


Home page
PediatricsHome page
Z. Zadik, T. Sinai, A. Zung, and R. Reifen
Effect of Nutrition on Growth in Short Stature Before and During Growth-Hormone Therapy
Pediatrics, July 1, 2005; 116(1): 68 - 72.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
N. D. Quinton, R. F. Smith, P. E. Clayton, M. S. Gill, S. Shalet, S. K. Justice, S. A. Simon, S. Walters, M.-C. Postel-Vinay, A. I. F. Blakemore, et al.
Leptin Binding Activity Changes with Age: The Link between Leptin and Puberty
J. Clin. Endocrinol. Metab., July 1, 1999; 84(7): 2336 - 2341.
[Abstract] [Full Text]


Home page
J. Clin. Endocrinol. Metab.Home page
S. Fisker, N. Vahl, J. O. L. Jorgensen, J. S. Christiansen, and H. Orskov
Abdominal Fat Determines Growth Hormone-Binding Protein Levels in Healthy Nonobese Adults
J. Clin. Endocrinol. Metab., January 1, 1997; 82(1): 123 - 128.
[Abstract] [Full Text] [PDF]


Home page
Arch Pediatr Adolesc MedHome page
M. Bozzola, K. Tettoni, F. Locatelli, G. Radetti, C. Belloni, M. Autelli, M. Zecca, R. Valentini, F. Severi, and L. Tato
Postnatal Variations of Growth Hormone Bioactivity and of Growth Hormone-Dependent Factors
Arch Pediatr Adolesc Med, October 1, 1996; 150(10): 1068 - 1071.
[Abstract] [PDF]


Home page
NEJMHome page
A. D. Goddard, R. Covello, S.-M. Luoh, T. Clackson, K. M. Attie, N. Gesundheit, A. C. Rundle, J. A. Wells, L. M.S. Carlsson, and The Growth Hormone Insensitivity Study Group
Mutations of the Growth Hormone Receptor in Children with Idiopathic Short Stature
N. Engl. J. Med., October 26, 1995; 333(17): 1093 - 1098.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1990 by The Endocrine Society