This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by GEFFNER, M. E. Articles by GOLDE, D. W. Search for Related Content PubMed PubMed Citation Articles by GEFFNER, M. E. Articles by GOLDE, D. W.

Growth Hormone Mediates the Growth of TLymphoblast Cell Lines via Locally Generated Insulin-Like Growth Factor-I^*

Departments of Pediatrics (M.E.G., B.M.L.) and Medicine (N.B., D.W.G.), University of California- Los Angeles Medical Center Los Angeles, California 90024
The Department of Pediatrics, Stanford University Medical Center (R.G.R., R.L.H.) Stanford, California 94305

Address requests for reprints to: Mitchell E. Geffner, M.D., Department of Pediatrics, University of California-Los Angeles Medical Center, Los Angeles, California 90024.

It has become evident that locally produced insulin-like growth factors-I and -II (IGF-I and IGF-II) play an important role in the mediation of GH action upon tissues. To explore this concept with respect to immunocompetent cells, we analyzed IGF production and clonogenic responsiveness of immortalized human T-cell lines established from seven normal controls and four Laron dwarfs. While the normal T-cell lines showed significant augmentation of basal colony formation in response to both IGF-I and GH, little increase in clonogenesis in response to GH was seen with the Laron T-cell lines. Assay of basal and GH-stimulated conditioned media demonstrated low, but measurable, levels of IGF-I and IGF-II from both normaland Laron T-cells. Under serum-free incubation conditions, GH stimulation of normal T-cell lines failed to generate significant increases in mean IGF-I or IGF-II concentrations. Only a marginal increase in the mean IGF-I concentration and no increase in the mean IGF-II concentration in conditioned medium were observed after GH stimulation of Laron T-cell lines. Nevertheless, the increased cloning efficiency of the normal T-cell lines in response to either GH or IGF-I was nearly completely abrogated by preincubation of cells with antibodies to either IGF-I or the type I IGF receptor. These studies, thus, support a role for locally generated IGF-I in the mediation of GH action on Tlymphocytes and indicate that this effect is mediated via the type I IGF receptor.

^* This work was supported in part by Grants DK-28229 (to R.G.R.), DK-36054 (to R.G.R.), DK-01275 (to R.G.R.), CA-42106 (to R.G.R.), DK-24085 (to R.L.H.), CA-32737 (to D.W.G.), and CA-30388 (D.W.G.) from the NIH.

Received January 22, 1990.

This article has been cited by other articles:

				L. A. Welniak, R. Sun, and W. J. Murphy The role of growth hormone in T-cell development and reconstitution J. Leukoc. Biol., March 1, 2002; 71(3): 381 - 387. [Abstract] [Full Text] [PDF]

				E. Baixeras, S. Jeay, P. A. Kelly, and M.-C. Postel-Vinay The Proliferative and Antiapoptotic Actions of Growth Hormone and Insulin-Like Growth Factor-1 Are Mediated through Distinct Signaling Pathways in the Pro-B Ba/F3 Cell Line Endocrinology, July 1, 2001; 142(7): 2968 - 2977. [Abstract] [Full Text] [PDF]

				B. Langkamp-Henken, K. A. Herrlinger-Garcia, J. K. Stechmiller, J. A. Nickerson-Troy, B. Lewis, and L. Moffatt Arginine Supplementation Is Well Tolerated but Does Not Enhance Mitogen-Induced Lymphocyte Proliferation in Elderly Nursing Home Residents with Pressure Ulcers JPEN J Parenter Enteral Nutr, September 1, 2000; 24(5): 280 - 287. [Abstract] [PDF]

				B. Lewis and B. Langkamp-Henken Arginine Enhances In Vivo Immune Responses in Young, Adult and Aged Mice J. Nutr., July 1, 2000; 130(7): 1827 - 1830. [Abstract] [Full Text]

				S. B. Solerte, M. Fioravanti, G. Vignati, A. Giustina, L. Cravello, and E. Ferrari Dehydroepiandrosterone Sulfate Enhances Natural Killer Cell Cytotoxicity in Humans Via Locally Generated Immunoreactive Insulin-Like Growth Factor I J. Clin. Endocrinol. Metab., September 1, 1999; 84(9): 3260 - 3267. [Abstract] [Full Text]

				S. Jain, D. W. Golde, R. Bailey, and M. E. Geffner Insulin-Like Growth Factor-I Resistance Endocr. Rev., October 1, 1998; 19(5): 625 - 646. [Abstract] [Full Text]

				M.-C. Postel-Vinay, V. de Mello Coelho, M.-C. Gagnerault, and M. Dardenne Growth Hormone Stimulates the Proliferation of Activated Mouse T Lymphocytes Endocrinology, May 1, 1997; 138(5): 1816 - 1820. [Abstract] [Full Text] [PDF]

				R. Clark The Somatogenic Hormones and Insulin-Like Growth Factor-1: Stimulators of Lymphopoiesis and Immune Function Endocr. Rev., April 1, 1997; 18(2): 157 - 179. [Abstract] [Full Text]

				P. S. Hinton, C. A. Peterson, H.-C. Lo, Huan Yang, D. Mccarthy, and D. M. Ney Insulin-Like Growth Factor-I Enhances Immune Response in Dexamethasone-Treated or Surgically Stressed Rats Maintained With Total Parenteral Nutrition JPEN J Parenter Enteral Nutr, November 1, 1995; 19(6): 444 - 452. [Abstract] [PDF]