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Expression of Messenger Ribonucleic Acid Species Encoding Steroidogenic Enzymes in Human Follicles and Corpora Lutea throughout the Menstrual Cycle^*

KEVIN J. DOODY, MATTHEW C. LORENCE, J. IAN MASON and EVAN R. SIMPSON

Cecil H. and Ida Green Center for Reproductive Biology Sciences, Departments of Obstetrics-Gynecology Biochemistry, University of Texas Southwestern Medical Center Dallas, Texas 75235

Address all correspondence and requests for reprints to: Dr. Evan Simpson, Cecil H. and Ida Green Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235–9051.

The levels of expression of mRNA species encoding cholesterol side-chain cleavage cytochrome P-450 (P450_SCC), 17-hydroxylase cytochrome P450 (P450₁₇), aromatase cytochrome P-450 (P-450_AROM), and 3β-hydroxysteroid dehydrogenase (3βHSD) were examined in human follicles and corpora lutea (CL) throughout the menstrual cycle. Tissues were obtained from women undergoing hysterectomy and oophorectomy. The largest follicle or the CL was dissected from the ovary depending on whether the surgery was performed in the follicular or luteal phase. The day of the cycle was determined by onset of last menstrual period and was confirmed by endometrial histology. Total RNA was examined by Northern blot analysis, using as probes specific ³²P-labeled cDNA inserts encoding each human enzyme. Early follicles demonstrated detectable mRNA for both P450_BCC and P450₁₇, but not for P450_AROM or 3βHSD. P450_AROM was detectable late in the follicular phase and appeared markedly induced in the CL. 3βHSD was detectable only in the CL. Levels of P450₁₇ mRNA remained relatively unchanged throughout the cycle, whereas P450_BCC mRNA levels were greatly increased in the CL. The presence of P450₁₇ mRNA in the human CL is of interest, since it is absent from the bovine CL, and this is consistent with the ability of the human, but not the bovine, CL to synthesize 17-hydroxyprogesterone and estrogens. The fact that P450_AROM expression is highest in CL is surprising, since plasma estrogen levels are highest during the late follicular phase of the cycle, and may suggest that CL estrogen biosynthesis is limited by 17-hydroxylase or 17,20-lyase activities. (J Clin Endocrinol Metab 70: 1041–1045, 1990)

^* This work was supported in part by USPHS Grants HD-13234, DK-28350, and AG-08175.

Supported in part by USPHS Training Grant 5-T32-HD-07190.

Received September 25, 1989.

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