Journal of Clinical Endocrinology & Metabolism, Vol 69, 1305-1308, Copyright © 1989 by Endocrine Society

ARTICLES

Plasma pancreastatin-like immunoreactivity in various diseases

K Tateishi, A Funakoshi, H Wakasugi, H Iguchi, H Shinozaki, M Abe, S Funakoshi, H Tamamura, H Yajima and Y Matsuoka
First Department of Biochemistry, Fukuoka University School of Medicine, Japan.

Plasma pancreastatin (PST)-like immunoreactivity in normal subjects and patients with various diseases was estimated by a RIA, using antiserum raised against a synthetic C-terminal peptide of human PST deduced from the sequence of human chromogranin-A. The mean level +/- SEM was 13.2 +/- 0.6 pmol/L in normal subjects, but was significantly higher in patients with chronic renal failure (526.7 +/- 48.5). An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A- (250-301)] and a larger form were detected by gel filtration of plasma from these patients, suggesting accumulation of the larger molecular form in these patients. A significant increase in PST-like immunoreactivity was also found in patients with liver cirrhosis (20.8 +/- 3.0 pmol/L), but not in patients with noninsulin-dependent diabetes mellitus, chronic pancreatitis, or pancreatic cancer. Elevated levels were found in 16 of the 21 patients with small cell lung carcinoma examined. High levels were also found in 3 of 11 patients with islet cell tumor.