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Half-Time of Endogenous Growth Hormone (GH) Disappearance in Normal Man After Stimulation of GH Secretion by GH-Releasing Hormone and Suppression with Somatostatin^*

AMILTON C. S. FARIA, JOHANNES D. VELDHUIS, MICHAEL O. THORNER and MARY LEE VANCE

Department of Internal Medicine, University of Virginia Medical School Charlottesville, Virginia 22908

Address all correspondence and requests for reprints to: Mary Lee Vance, M.D., Box 511, University of Virginia Medical Center, Charlottesville, Virginia 22908.

The half-life (t) of disappearance of endogenous GH from serum was studied using physiological effectors to stimulate and then suppress GH release. GH secretion was stimulated by a single iv injection of GHRH, followed 45 min later by an iv bolus dose and then a 2.5-h infusion of somatostatin (SRIH) to suppress further release. The in vivo t of GH in seven men was calculated from serum GH concentrations measured at frequent intervals after beginning the SRIH infusion. The mean t of endogenous GH was 18.9 ± 0.8 (±SE) min by monoexponential analysis and 3.5 ± 0.7 and 20.7 ± 0.7 min by biexponential fitting. In these normal men, the decline in GH concentrations after GHRH and SRIH administration was similar to that after the administration of GHRH alone, which yielded a t of 20.3 ± 1.9 min. We conclude that the physiological kinetics of endogenous GH removal/disappearance can be estimated in vivo in man using GHRH with or without SRIH infusion.

^* This work was supported by USPHS Grant RR-00847 (to the GCRC), Research Career Development Award 1K041400634 (to J.D.V.), and Grant DK-32632 (to M.O.T.).

Visiting Fellow, supported by CAPES (Post-Graduate Education Federal Agency), Brazil.

Received July 29, 1988.

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