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Analysis of Hormone Secretion by Clinically Nonfunctioning Human Pituitary Adenomas Using the Reverse Hemolytic Plaque Assay^*

SHOZO YAMADA, SYLVIA L. ASA, KALMAN KOVACS, PAUL MULLER and HARLEY S. SMYTH

Departments of Pathology (S.Y., S.L.A., K.K.) and Neurosurgery (P.M.), St. Michael’s Hospital, and the Department of Neurosurgery, Wellesley Hospital (H.S.S.), University of Toronto Toronto,Ontario, M5B 1W8 Canada

Address all correspondence and requests for reprints to: S. L. Asa, M.D., Department of Pathology, St. Michael’s Hospital, 30 Bond Street, Toronto, Ontario, M5B 1W8 Canada.

The reverse hemolytic plaque assay was used to study hormone release in vitro by seven clinically nonfunctioning human pituitary adenomas associated with no clinical or biochemical evidence of hormone excess. Four of seven tumors were oncocytomas, one a null cell adenoma, and two gonadotroph adenomas based on immunocytochemical and ultrastructural features. In all seven tumors, plaques were formed with antiserum against βFSH; four produced plaques for βLH, and five for glycoprotein hormone -subunit. The percentageof plaqueforming cells and the mean size of plaques were smaller thanthose of clinically functioning adenomas studied for comparison (five GH- and/or PRL-producing adenomas).These results correlated with those of hormone release in tissue culture, immunocytochemistry on paraffin secretions of the tumors, and immunocytochemistry after reverse hemolytic plaque assay. We conclude that clinically nonfunctioning pituitary adenomas release small quantities of hormones, primarily gonadotropins, and that hormone release is attributable to only a small percentage of tumor cells.

^* This work was supported in part by the Connaught Foundation, the Dean’s Fund of the University of Toronto, Faculty of Medicine,the St. Michael’s Hospital Research Society, and Grants MA-10215 and MT-6349 awarded by the Medical Research Council of Canada.

Supported by the Toranomon Hospital and the Okinaka Memorial Institute of Medical Research, Tokyo, Japan.

Received April 22, 1988.

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