Journal of Clinical Endocrinology & Metabolism, Vol 67, 1250-1257, Copyright © 1988 by Endocrine Society

ARTICLES

Different insulin-like growth factor binding species in human placenta and decidua

F Pekonen, AM Suikkari, T Makinen and EM Rutanen
Minerva Institute for Medical Research, Kauniainen, Finland.

Previous studies demonstrated that human decidua secretes a 34K insulin- like growth factor binding protein (34K IGF-BP) earlier designated placental protein 12, whereas placenta contains IGF receptors and IGF mRNA. We studied binding of [125I]IGF-I to paired placental and decidual tissues using competitive binding, gel filtration, RIA, and cross-linking techniques, and compared the binding characteristics of these two tissues which are located in close proximity in vivo. The effect of decidual cytosols on [125I]IGF-I binding to placental membranes also was studied. Consistent with previous data the dominating binding species in placental membranes were IGF receptors. In contrast, the binding of [125I]IGF-I to decidual membranes was mainly due to binding species with mol wts of 34K and 39K. The 34K IGF- BP was more readily detected in decidual cytosols than in decidual membranes and little was detected in placental cytosols. Purified 34K IGF-BP as well as decidual cytosols inhibited [125I]IGF-I binding to placental receptors. The inhibitory effect of decidual cytosol on IGF receptor binding was linearly correlated to the decidual content of 34K IGF-BP. The results suggest that the decidual 34K IGF-BP might act as a local modulator of the IGF action at the interface between the decidua and the placenta.

This article has been cited by other articles:

				F A Hills, M G Elder, T Chard, and M H F Sullivan Regulation of human villous trophoblast by insulin-like growth factors and insulin-like growth factor-binding protein-1 J. Endocrinol., December 1, 2004; 183(3): 487 - 496. [Abstract] [Full Text] [PDF]

				A. Mero, J. Kahkonen, T. Nykanen, T. Parviainen, I. Jokinen, T. Takala, T. Nikula, S. Rasi, and J. Leppaluoto IGF-I, IgA, and IgG responses to bovine colostrum supplementation during training J Appl Physiol, August 1, 2002; 93(2): 732 - 739. [Abstract] [Full Text] [PDF]

				N. Anim-Nyame, F.A. Hills, S.R. Sooranna, P.J. Steer, and M.R. Johnson A longitudinal study of maternal plasma insulin-like growth factor binding protein-1 concentrations during normal pregnancy and pregnancies complicated by pre-eclampsia Hum. Reprod., October 1, 2000; 15(10): 2215 - 2219. [Abstract] [Full Text] [PDF]

				R. P Holmes, J M P Holly, and P. W Soothill Maternal insulin-like growth factor binding protein-1, body mass index, and fetal growth Arch. Dis. Child. Fetal Neonatal Ed., March 1, 2000; 82(2): 113F - 117. [Abstract] [Full Text]

				P. Caron, L. Buscail, A. Beckers, J.-P. Esteve, A. Igout, G. Hennen, and C. Susini Expression of Somatostatin Receptor SST4 in Human Placenta and Absence of Octreotide Effect on Human Placental Growth Hormone Concentration during Pregnancy J. Clin. Endocrinol. Metab., November 1, 1997; 82(11): 3771 - 3776. [Abstract] [Full Text] [PDF]