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Immunocytochemical Detection of 1,25-Dihydroxyvitamin D Receptors in Normal Human Tissues^*

Ludwig Institute for Cancer Research (U.B., P. W., R.A.M., R.C.C.) and the Chemical Pathology Department (K.C.), St. George's Hospital Medical School London, SW17 ORE United Kingdom
the Department of Biochemistry, University of Arizona (M.R.H.), Tuscon, Arizona 85724; and Baylor College of Medicine (J. W.P.) Houston, Texas 77054

Address requests for reprints to: Dr. Uta Berger, Ludwig Institute of Cancer Research, St. George's Hospital, Cranmer Terrace, London, SW17 ORE United Kingdom.

We developed an immunocytochemical technique to visualize the receptors for 1,25-dihydroxyvitamin D[1,25-(OH)₂D receptor] in cryostat sections of normal human tissues, using a rat monoclonal antibody (9A7) to the chick intestinal receptor, which has been found to react with mammalian 1,25-(OH)₂D receptors. Localization of the antigen was predominantly nuclear, with little cytoplasmic immunoreactivity. Specific staining was seen in the nuclei of many normal epithelial tissues, including liver, kidney, thyroid, adrenal, gastrointestinal tract, breast, and skin. No nuclear staining was seen when tissue sections were incubated with normal rat immunoglobulin G or when the monoclonal antibody was preincubated with a receptor-enriched chick intestinal cytosol preparation. Our results demonstrate that the receptor for 1,25-(OH)₂D is localized in the nucleus and widely distributed in normal human tissues.

^* This work was supported in part by grants from the Cancer Research Campaign and the Pathological Research Fund, St. George's Hospital Medical School.

Received November 17, 1987.

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