This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by BINOUX, M. Articles by HOSSENLOPP, P. Search for Related Content PubMed PubMed Citation Articles by BINOUX, M. Articles by HOSSENLOPP, P.

Insulin-Like Growth Factor (IGF) and IGF-Binding Proteins: Comparison of Human Serum and Lymph^*

MICHEL BINOUX and PAUL HOSSENLOPP

INSERM U142, Hôpital Trousseau 75012 Paris, France

The extent to which the association between insulin-like growth factors (IGFs) and their specific binding proteins (BPs) prevents their crossing the capillary barrier was studied by comparing their distribution in serum with that in samples of lymph collected from the lower leg of five subjects undergoing radiographical investigation of the lymphatic system. The IGF concentrations in lymph were 10–30' of the corresponding serum levels, and in each subject the ratios of IGF-I and IGF-II in the lymph to those in the serum were similar. Western blot analysis of the BPs revealed that the five molecular forms identified in serum also were present in lymph, but in significantly smaller quantities. The 41.5K and 38.5K forms, which constitute the binding units of the large complex (150K) of serum and are also capable of binding IGFs in monomeric form, were present in smaller amounts than the 34K, 30K and 24K forms, which belong specifically to the small complex (40K) of serum. The BPs extracted from lymph were similar to those of the small complex, with a preferential affinity for IGF-II and only half of the affinity for IGF-I of the BPs extracted from serum. With neutral pH gel filtration of lymph, more than 90% of IGFs and binding activity eluted with the material in the area of the 40K zone.

These data indicate that the 150K IGF-BP complexes do not cross the capillary barrier, whereas the 40K complexes do. The function of the former may be to provide a reservoir and buffering action of the IGFs, whereas the latter may be involved in the transport of the IGFs to their target cells.

^* Presented in part at the International Congress on Advances in Growth Hormone and Growth Factors Research, Milan, Italy, September 28-30,1987. This work was supported by INSERM.

To whom requests for reprints should be addressed.

Received December 28, 1987.

This article has been cited by other articles:

				M. A. Nauck, M. Reinecke, A. Perren, J. Frystyk, G. Berishvili, C. Zwimpfer, A. M. Figge, A. Flyvbjerg, P. G. Lankisch, W. F. Blum, et al. Hypoglycemia due to Paraneoplastic Secretion of Insulin-Like Growth Factor-I in a Patient with Metastasizing Large-Cell Carcinoma of the Lung J. Clin. Endocrinol. Metab., May 1, 2007; 92(5): 1600 - 1605. [Abstract] [Full Text] [PDF]

				J. W Kim, R. P Rhoads, N. Segoale, N. B Kristensen, D. E Bauman, and Y. R Boisclair Isolation of the cDNA encoding the acid labile subunit (ALS) of the 150 kDa IGF-binding protein complex in cattle and ALS regulation during the transition from pregnancy to lactation. J. Endocrinol., June 1, 2006; 189(3): 583 - 593. [Abstract] [Full Text] [PDF]

				D. A. M. Salih, G. Tripathi, C. Holding, T. A. M. Szestak, M. I. Gonzalez, E. J. Carter, L. J. Cobb, J. E. Eisemann, and J. M. Pell Insulin-like growth factor-binding protein 5 (Igfbp5) compromises survival, growth, muscle development, and fertility in mice PNAS, March 23, 2004; 101(12): 4314 - 4319. [Abstract] [Full Text] [PDF]

				S. M. Firth and R. C. Baxter Cellular Actions of the Insulin-Like Growth Factor Binding Proteins Endocr. Rev., December 1, 2002; 23(6): 824 - 854. [Abstract] [Full Text] [PDF]

				S.-A. Wandji, J. E. Gadsby, F. A. Simmen, J. A. Barber, and J. M. Hammond Porcine Ovarian Cells Express Messenger Ribonucleic Acids for the Acid-Labile Subunit and Insulin-Like Growth Factor Binding Protein-3 during Follicular and Luteal Phases of the Estrous Cycle Endocrinology, July 1, 2000; 141(7): 2638 - 2647. [Abstract] [Full Text] [PDF]

				K. R. Kritsch, D. J. Huss, and D. M. Ney Greater potency of IGF-I than IGF-I/BP-3 complex in catabolic parenterally fed rats Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E252 - E262. [Abstract] [Full Text] [PDF]

				T. Sakabe, T. Shinomiya, T. Mori, Y. Ariyama, Y. Fukuda, T. Fujiwara, Y. Nakamura, and J. Inazawa Identification of a Novel Gene, MASL1 within an Amplicon at 8p23.1 Detected in Malignant Fibrous Histiocytomas by Comparative Genomic Hybridization Cancer Res., February 1, 1999; 59(3): 511 - 515. [Abstract] [Full Text] [PDF]

				S. M. Twigg, M. C. Kiefer, J. Zapf, and R. C. Baxter Insulin-like Growth Factor-binding Protein 5 Complexes with the Acid-labile Subunit. ROLE OF THE CARBOXYL-TERMINAL DOMAIN J. Biol. Chem., October 30, 1998; 273(44): 28791 - 28798. [Abstract] [Full Text] [PDF]

				J. Frystyk, H. Gronbak, C. Skjarbak, A. Flyvbjerg, H. Orskov, and R. C. Baxter Developmental Changes in Serum Levels of Free and Total Insulin-Like Growth Factor I (IGF-I), IGF-Binding Protein-1 and -3, and the Acid-Labile Subunit in Rats Endocrinology, October 1, 1998; 139(10): 4286 - 4292. [Abstract] [Full Text] [PDF]

				T. Seck, B. Scheppach, S. Scharla, I. Diel, W. F. Blum, H. Bismar, G. Schmid, B. Krempien, R. Ziegler, and J. Pfeilschifter Concentration of Insulin-Like Growth Factor (IGF)-I and -II in Iliac Crest Bone Matrix from Pre- and Postmenopausal Women: Relationship to Age, Menopause, Bone Turnover, Bone Volume, and Circulating IGFs J. Clin. Endocrinol. Metab., July 1, 1998; 83(7): 2331 - 2337. [Abstract] [Full Text]

				A. Sandra, M. Boes, B. L. Dake, J. B. Stokes, and R. S. Bar Infused IGF-I/IGFBP-3 complex causes glomerular localization of IGF-I in the rat kidney Am J Physiol Endocrinol Metab, July 1, 1998; 275(1): E32 - E37. [Abstract] [Full Text] [PDF]

				G. T. Ooi, K. R. Hurst, M. N. Poy, M. M. Rechler, and Y. R. Boisclair Binding of STAT5a and STAT5b to a Single Element Resembling a {gamma}-Interferon-Activated Sequence Mediates the Growth Hormone Induction of the Mouse Acid-Labile Subunit Promoter in Liver Cells Mol. Endocrinol., May 1, 1998; 12(5): 675 - 687. [Abstract] [Full Text]

				D. R. Powell, S. K. Durham, F. Liu, B. K. Baker, P. D. K. Lee, S. L. Watkins, P. G. Campbell, E. D. Brewer, R. L. Hintz, and R. J. Hogg The Insulin-Like Growth Factor Axis and Growth in Children with Chronic Renal Failure: A Report of the Southwest Pediatric Nephrology Study Group J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1654 - 1661. [Abstract] [Full Text]

				S. M. Twigg and R. C. Baxter Insulin-like Growth Factor (IGF)-binding Protein 5 Forms an Alternative Ternary Complex with IGFs and the Acid-labile Subunit J. Biol. Chem., March 13, 1998; 273(11): 6074 - 6079. [Abstract] [Full Text] [PDF]

				R. E. Drake, R. A. Teague, and J. C. Gabel Effect of sheep plasma and lymph on fibroblast proliferation Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R561 - R563. [Abstract] [Full Text] [PDF]

				S. Rajaram, D. J. Baylink, and S. Mohan Insulin-Like Growth Factor-Binding Proteins in Serum and Other Biological Fluids: Regulation and Functions Endocr. Rev., December 1, 1997; 18(6): 801 - 831. [Abstract] [Full Text]

				E. Gay, D. Seurin, S. Babajko, S. Doublier, M. Cazillis, and M. Binoux Liver-Specific Expression of Human Insulin-Like Growth Factor Binding Protein-1 in Transgenic Mice: Repercussions on Reproduction, Ante- and Perinatal Mortality and Postnatal Growth Endocrinology, July 1, 1997; 138(7): 2937 - 2947. [Abstract] [Full Text] [PDF]

				G. T. Ooi, F. J. Cohen, L. Y.-H. Tseng, M. M. Rechler, and Y. R. Boisclair Growth Hormone Stimulates Transcription of the Gene Encoding the Acid-Labile Subunit (ALS) of the Circulating Insulin-Like Growth Factor-Binding Protein Complex and ALS Promoter Activity in Rat Liver Mol. Endocrinol., June 1, 1997; 11(7): 997 - 1007. [Abstract] [Full Text]

				C. Y. Lee, H.-B. Wu, D.-S. Suh, and M. M. Rechler Insulin-Like Growth Factor-I (IGF-I) Concentration in 150-Kilodalton Complexes Containing Human IGF-Binding Protein-3 (hIGFBP-3) after Intravenous Injection of Adult Rats with hIGFBP-3 Endocrinology, April 1, 1997; 138(4): 1649 - 1657. [Abstract] [Full Text] [PDF]

				M. Westwood, J M. Gibson, and A. White Purification and Characterization of the Insulin-Like Growth Factor-Binding Protein-1 Phosphoform Found in Normal Plasma Endocrinology, March 1, 1997; 138(3): 1130 - 1136. [Abstract] [Full Text] [PDF]