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Phenotypic Diversity of the Cellular 1,25-Dihydroxyvitamin D₃-Receptor Interaction Among Different Genera of New World Primates

University of Southern California/Orthopaedic Hospital, Endocrine Research Laboratory Los Angeles, California 90007

Address all correspondence and requests for reprints to: Dr. John S. Adams, Department of Medicine, University of Southern California School of Medicine, 5th Floor, D and T Building, 2400 South Flower Street, Los Angeles, California 90007.

High serum of 1,25-dihydroxyvitamin D₃ [1,25-(OH)₂D₃] concentrations and target organ resistance to the hormone are characteristic findings in some New World primates (platyrrhines). We examined the abilities of cultural dermal fibroblasts from individual platyrrhines of four different genera, Aotus, Alouatta, Pithecia, and Saguinus, to internalize and respond to 1,25-(OH)₂D₃ in an attempt to identify possible phenotypic heterogeneity in the l,25-(OH)₂D₃-receptor interaction among them. Results were compared to those from two Old World primates (catarrhines), Pan troglodytes and Pongo pygmaeus. Compared to catarrhine cells, cells from Alouatta, Pithecia, and Saguinus demonstrated 1) a 10-fold decrease in [³H] 1,25-(OH)₂D₃ internalization capacity; 2) a 2- to 5-fold increase in the apparent internalization constant [³H]1,25-(OH)₂D₃; and 3) a 3- to 15-fold increase in the 1,25-(OH)₂D₃ concentration required to elicit half-maximal induction of [³H]25-hydroxyvitamin D₃-24-hydroxylating activity (ED₅₀; rank order Sanguinus >> Pithecia > Alouatta). Although the internalization capacity of cells from two different primates in the genus Aotus was 3- to 4-fold lower than that in catarrhine cells, the internalization constant for hormone and ED₅₀ for 24-hydroxylating activity were similar. These data suggest that the functional 1,25-(OH)₂D₃-receptor phenotype of the owl monkey, Aotus triuirgatus, is more closely aligned to the catarrhine phenotype than are those of other platyrrhines in the families Cebidae and Callitricidae.

Received June 26, 1987.

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