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Journal of Clinical Endocrinology & Metabolism, Vol 65, 868-875, Copyright © 1987 by Endocrine Society

ARTICLES

Antiserum developed for the E peptide region of insulin-like growth factor IA prohormone recognizes a serum protein by both immunoblot and radioimmunoassay

DR Powell, PD Lee, D Chang, F Liu and RL Hintz
Department of Pediatrics, Stanford University Medical Center, California 94305.

Nucleotide sequencing of the two known cDNAs encoding human insulin- like growth factor I (IGF-I) predicts two different prohormone forms of IGF-I. The predicted prohormone amino acid sequences (E peptide regions) extend the carboxy-terminus of IGF-I by either an additional 35 (IGF-IA) or 77 (IGF-IB) amino acids. We developed an antiserum directed against a synthetic peptide which is unique to the E peptide region of IGF-IA prohormone. In a RIA using this antiserum, synthetic E peptide immunoreactivity was found in the serum of patients with chronic renal failure. The protein recognized by this antiserum has a mol wt of about 13,000 by neutral gel filtration and about 19,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. These data suggest that the E peptide region of the IGF- IA prohormone is expressed and circulates as part of the prohormone.


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