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Journal of Clinical Endocrinology & Metabolism Vol. 65, No. 5 1014-1019
doi:10.1210/jcem-65-5-1014
Copyright © 1987 by the Endocrine Society.
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Evidence of Thyrotropin-Releasing Hormone (TRH) and TRH-Binding Sites in Human Nonsecreting Pituitary Adenomas

M. LE DAFNIET, D. GROUSELLE, J. Y. LI, M. KUJAS, D. BRESSION, A. BARRET, A. TIXIER-VIDAL and F. PEILLON

INSERM U. 223 (M.L.D., J.Y.L., D.B., F.P.) et Laboratoire d'Histologie-Embryologie (M.K.), Faculté de Médecine Pitié-Salpêtrière 75634 Paris Cedex 13, France
Groupe de Neuroendocrinologie Cellulaire, Collège de France (D.G., A.B., A.T.-V.) 75231 Paris, France

Address all correspondence and requests for reprints to: M. Le Dafniet, INSERM U. 223, Faculté de Médecine Pitié-Salpêtrière, 105 Boulevard de l'Hôpital, 75634 Paris Cedex 13, France.

Immunoreactive TRH (IR-TRH) and TRHbinding sites were sought in nonsecreting pituitary adenomas. [3H]TRH bound specifically to cellular membranes from 11 of 12 such adenomas studied, with a dissociation constant (Kd) of 50 ± 5 (±SEM) nmol/L and a maximum number of binding sites of 76 ± 16 fmol/mg membrane protein (range, 32-229 fmol/mg protein). IR-TRH was detected in all 8 of the tumors in which it was sought. The identity of the IR-TRH was verified by high pressure liquid chromatography. The tumor IR-TRH concentration varied from 45-248 fmol/mg cell protein (mean, 109 ± 28 fmol/mg cell protein), about half that in normal human pituitary 229 ± 55 fmol/mg protein). There was no correlation between the number of binding sites and the IR-TRH content. The role of TRH in nonsecreting pituitary adenomas is unknown at this time.

Received December 12, 1986.




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Copyright © 1987 by The Endocrine Society