Journal of Clinical Endocrinology & Metabolism, Vol 65, 747-752, Copyright © 1987 by Endocrine Society

ARTICLES

In vitro net progesterone production by human corpora lutea: effects of human chorionic gonadotropin, dibutyryl adenosine 3',5'-monophosphate, cholera toxin, and forskolin

M Vega, L Devoto, V Navarro, O Castro and P Kohen
Department of Basic Sciences, Faculty of Medicine, University of Chile, Santiago.

Slices of human corpora lutea (CL) obtained at varying stages of the luteal phase from 21 women were used to study the effect of hCG on progesterone (P4) production. Slices obtained from mid- and late CL incubated with 10 IU/mL hCG exhibited a significant increase in net P4 production (P less than 0.001), whereas slices from early CL did not. Mid-CL slices were the most sensitive to hCG (4.2-fold increase in P4 production compared to 1.2-fold for early CL and 2.7-fold for late CL). To investigate the unresponsiveness of early CL to hCG, [125I]hCG binding was studied. All early CL had LH/hCG-specific receptors, and the apparent Kd for this binding was 1.95 X 10(-10) M. Dibutyryl cAMP (1 mM), cholera toxin (0.84 mM), and forskolin (50 microM) stimulated net P4 production (P less than 0.05) in slices of early CL tissue incubated in the presence of methylisobutylxanthine (0.1 mM). Cholera toxin and forskolin stimulated cAMP formation by the early CL, but hCG failed to do so. These results confirm that hCG has an age-dependent stimulatory effect on CL P4 synthesis. Our findings suggest that there is inadequate coupling of the LH/hCG receptor and adenylate cyclase in the early human CL, which explains in part the relative insensitivity of this tissue to the steroidogenic action of hCG.

This article has been cited by other articles:

				D. Ghosh, S. Sachdev, M. Hannink, and R. M. Roberts Coordinate Regulation of Basal and Cyclic 5'-Adenosine Monophosphate (cAMP)-Activated Expression of Human Chorionic Gonadotropin-{alpha} by Ets-2 and cAMP-Responsive Element Binding Protein Mol. Endocrinol., April 1, 2005; 19(4): 1049 - 1066. [Abstract] [Full Text] [PDF]

				P. Kohen, O. Castro, A. Palomino, A. Munoz, L. K. Christenson, W. Sierralta, P. Carvallo, J. F. Strauss III, and L. Devoto The Steroidogenic Response and Corpus Luteum Expression of the Steroidogenic Acute Regulatory Protein after Human Chorionic Gonadotropin Administration at Different Times in the Human Luteal Phase J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3421 - 3430. [Abstract] [Full Text] [PDF]

				M. Vega, L. Urrutia, G. Iniguez, F. Gabler, L. Devoto, and M.C. Johnson Nitric oxide induces apoptosis in the human corpus luteum in vitro Mol. Hum. Reprod., August 1, 2000; 6(8): 681 - 687. [Abstract] [Full Text] [PDF]