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Journal of Clinical Endocrinology & Metabolism, Vol 65, 105-109, Copyright © 1987 by Endocrine Society

ARTICLES

Skin-derived fibroblasts respond to human parathyroid hormone-like adenylate cyclase-stimulating proteins

TL Wu, KL Insogna, LM Hough, L Milstone and AF Stewart

Human tumors and keratinocyte-conditioned medium contain PTH-like adenylate cyclase-stimulating proteins. Human dermal fibroblasts have receptors that recognize PTH and a factor associated with humoral hypercalcemia of malignancy in rats. We examined 10 human dermal fibroblast lines for an adenylate cyclase response to PTH. Six of 10 lines tested displayed a definite response (2.4- to 3.8-fold over basal) to 10(-6) M bovine PTH-(1-34). This response was inhibited by the PTH analog and antagonist Nle8,18,Tyr34-bPTH-(3-34). We also examined whether human dermal fibroblasts are capable of responding to either a human PTH-like tumor-derived factor or the PTH-like factor contained in human keratinocyte-conditioned medium. Both human humoral hypercalcemia of malignancy-associated tumor extract (2.5 X 10(-10) M) and keratinocyte-conditioned medium (8 X 10(-10) M) stimulated human dermal fibroblast adenylate cyclase. These concentrations are markedly lower than those required for PTH-induced adenylate cyclase stimulation. This activity was also inhibited by the PTH analog. The high prevalence of PTH-responsive adenylate cyclase in dermal fibroblast lines and the apparent potency of tumor-derived and keratinocyte-derived PTH-like factors in dermal fibroblasts suggest that these factors may play a role in normal dermal physiology.


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J. H. Shin, C. Ji, S. Casinghino, T. L. McCarthy, and M. Centrella
Parathyroid Hormone-related Protein Enhances Insulin-like Growth Factor-I Expression by Fetal Rat Dermal Fibroblasts
J. Biol. Chem., September 19, 1997; 272(38): 23498 - 23502.
[Abstract] [Full Text] [PDF]

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 Palliat MedHome page
D. Heath
Review article : Hypercalcaemia of malignancy
Palliative Medicine, January 1, 1989; 3(1): 1 - 11.
[Abstract] [PDF]


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