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Identification of Inhibin Secreted by Cynomolgus Monkey Sertoli Cell Cultures^*

Department of Medicine, Montefiore Hospital, and University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania 15213

Address requests for reprints to: Dr. Philip Troen, Department of Medicine, Montefiore Hospital, 3459 Fifth Avenue, Pittsburgh, Pennsylvania 15213.

An inhibin was identified in the media of primary Sertoli cell-enriched cultures from the cynomolgus monkey, Macaca fascicularis, and some of its biochemical properties were studied. Conditioned monkey Sertoli cell culture medium (m-SCCM), when added to pituitary cells from 6-week-old male rats, inhibited the basal secretion of FSH but not that of LH. This specificity was lost after the addition of GnRH; mSCCM inhibited not only FSH but also LH release, determined by both RIA and mouse interstitial cell bioassay, from pituitary cells exposed for 6 h to 10 nM GnRH. FSH-inhibiting activity persisted when m-SCCM was boiled for 30 min, but activity was lost after incubation for 1 h at 37 C with 0.1% trypsin. m-SCCM inhibin activity was completely retained by Concanavalin A-Sepharose and could be eluted with 0.2 M -methyl-D-glucoside. Gel filtration high pressure liquid chromatography with a Su-perose-12 column revealed inhibin activity between 20–60K, with the greatest activity at 40K. Our results indicate that primate Sertoli cells produce an inhibin-like factor which could play a role in controlling gonadotropin secretion in males.

^* A portion of these data was presented at the 67th Annual Meeting of The Endocrine Society, Baltimore, MD, June 1985, and the 11th Annual Meeting of the American Society of Andrology, Grand Rapids, MI, April 1986.

Received July 21, 1986.

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