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Journal of Clinical Endocrinology & Metabolism, Vol 64, 737-743, Copyright © 1987 by Endocrine Society

ARTICLES

Characteristics of hepatic receptors for somatomedin-C/insulin-like growth factor I and insulin in the developing human

SD Chernausek, DC Beach, W Banach and MA Sperling

Insulin and the somatomedins (Sms) are putative regulators of cell proliferation and metabolism in the fetus. Since the liver is a potential target tissue of these hormones during fetal life, we characterized the hepatic receptors for Sm-C/insulin like growth factor I (Sm-C/IGF-I) and insulin during the second trimester of human fetal development. Membrane-enriched fetal liver homogenates specifically bound 8.9 +/- 1.5% (+/- SD) of added [125I]insulin and 5.1-7.2% of [125I]Sm-C/IGF-I. Binding of both hormones was constant from 12-20 weeks gestation and was much greater than that in adult liver membranes. Analysis of dose-response data indicated high affinity between each receptor and its respective ligand (Kd for the Sm-C/IGF-I receptor, 2.2 X 10(-10) M; Kd for insulin receptor, 5.2 X 10(-10) and 7.7 X 10(-9) M). Limited cross-reactivity (approximately 1:1,000) of insulin with the Sm-C/IGF-I receptor and Sm-C/IGF-I with the insulin receptor was found. Affinity labeling studies showed that each receptor possessed an approximately 135,000-dalton subunit which was a part of a larger disulfide-linked complex. Thus, the human fetal liver has specific receptors for Sm-C/IGF-I and insulin that are similar to those described for other tissues in terms of both hormone-binding characteristics and subunit structure, suggesting that these receptors mediate important cellular functions at this stage of fetal development.


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A. L. Ogilvy-Stuart, M. A. Soos, S. J. Hands, M. Y. Anthony, D. B. Dunger, and S. O'Rahilly
Hypoglycemia and Resistance to Ketoacidosis in a Subject without Functional Insulin Receptors
J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3319 - 3326.
[Abstract] [Full Text] [PDF]


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Copyright © 1987 by The Endocrine Society