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Journal of Clinical Endocrinology & Metabolism Vol. 64, No. 4 692-697
doi:10.1210/jcem-64-4-692
Copyright © 1987 by the Endocrine Society.
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Studies on Adrenorphin in Pheochromocytoma*

TOSHIHIKO YANASE, HAJIME NAWATA, KEN-ICHI KATO, HIROSHI IBAYASHI and HISAYUKI MATSUO

Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Fukoka 812
The Department of Biochemistry, Miyazaki Medical College Kiyotake, Miyazaki 889–16, Japan

Address requests for reprints to: Dr. Toshihiko Yanase, Third Department of Internal Medicine, Kyushu University, Fukuoka 812, Japan.

We studied the secretion and tissue contents of adrenorphin in human pheochromocytomas. In 17 human pheo-chromocytomas from 11 patients, we found a remarkably wide distribution in immunoreactive adrenorphin levels (3–7771 pg/mg tissue). Adrenormedullary pheochromocytomas contained a significantly larger amount of immunoreactive adrenorphin (2295 ± 1092 pg/mg, mean ± SE) than did extramedullary ones (17.8 ± 8.4 pg/mg). Gel chromatographic studies revealed that immunoreactive adrenorphin consisted largely of material emerging at the position of synthetic adrenorphin in both phe-ochromocytoma and normal adrenal medulla tissue. Nicotine (10–5 M) significantly stimulated the secretion of immunoreac-tive adrenorphin as well as catecholamines from cultured human pheochromocytoma cells. Adrenorphin was a more potent inhibitor of catecholamine secretion evoked by 10–5 M nicotine than was met-enkephalin in cultured human pheochromocytoma 6 cells. The 50% inhibitory concentrations (IC50) were 1.1 x 10–6 and 6.5 x 10–5 M for adrenorphin and met-enkephalin, respectively. The effect of adrenorphin was much the same as that of dynorphin-(1–13) (IC50) 1.0 x 10–6 M) and BAM-12P (IC50, 4.5 x 10–6 M).

These results indicate the presence and secretion of adrenorphin in human pheochromocytomas. Adrenorphin may play an important role in regulating catecholamine secretion in human pheochromocytoma.

* This work was supported in part by a Grant-in Aid for Special Project Research of Selected Intractable Neurological Disorders, Ministry of Education and a research grant from the Research Committee of Adrenal Hormone Disorders, Ministry of Health and Welfare, Japan.

Received July 15, 1986.







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Copyright © 1987 by The Endocrine Society