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Department of Pediatrics, Stanford University School of Medicine Stanford, California 94305
The structural and immunological properties of the insulin-like growth factor II (IGF-II) receptor on IM-9 lymphoblasts were studied using a combination of competitive binding and affinity cross-linking techniques as well as with a panel of polyclonal and monoclonal antireceptor antibodies. Unlike (IGF-II) binding to the classical type II IGF receptor, [125I] IGF-II binding to IM-9 cells was potently inhibited not only by unlabeled IGF-II, but also by insulin (50Ü inhibition of binding at 2.5 and 1.5 nM, respectively). Affinity cross-linking of [125I] IGF-II to intact cells, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, demonstrated that the over-whelming majority of IGF-II binding was to a type I receptor (apparent mol wt, >300,000 unreduced and 135,000 reduced), with minimal binding to a type II receptor (apparent mol wt,220,000 unreduced and 240,000 reduced). After preincubation with five different antireceptor antibodies, inhibition of [126] IIGF-II binding was comparable to inhibition of [125I]insulinbinding. These studies demonstrate that in IM-9 cells, the majority of IGF-II binding is to a type I receptor with high affinity for both insulin and IGF-II. Whether this is an atypical insulinreceptor or a unique type I receptor remains to be established.
* This work was supported in part by Grants AM-24085 (to R.L.H.) and AM-28229 (to R.G.R.) from the NIH.
Recipient of Research Career Development Award AM-01275 from the USPHS. To whom all correspondence and requests for reprints should be addressed.
Received January 13, 1986.
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