Journal of Clinical Endocrinology & Metabolism, Vol 63, 789-791, Copyright © 1986 by Endocrine Society

ARTICLES

Assignment of the gene for adrenal P450c17 (steroid 17 alpha- hydroxylase/17,20 lyase) to human chromosome 10

KJ Matteson, J Picado-Leonard, BC Chung, TK Mohandas and WL Miller

P450c17 is the single enzyme mediating both 17 alpha-hydroxylase and 17,20 lyase activities. We identified several human P450c17 cDNA clones in a human adrenal cDNA library we constructed in lambda gt10. A short clone containing the 3'-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450c17 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450c17 gene lies on chromosome 10. The chromosomal locations of the other P450c17 genes could not be determined.

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