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Journal of Clinical Endocrinology & Metabolism Vol. 63, No. 1 192-196
doi:10.1210/jcem-63-1-192
Copyright © 1986 by the Endocrine Society.
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Lack of Inhibitory Effect of {alpha}-Human Atrial Natriuretic Polypeptide on Aldosteronogenesis in Aldosterone-Producing Adenoma*

KAZUMI HIGUCHI, HAJME NAWATA, KEN-ICHI KATO, HIROSHI IBAYASHI and HISAYUKI MATSUO

Third Department of Internal Medicine, Faculty of Medicine, Kyushu University Fukuoka 812 Department of Biochemistry, Miyazaki Medical College Kiyotake, Miyazaki 889-16, Japan

Address requests for reprints to: Dr. Kazumi Higuchi, Third Department of Internal Medicine, Kyushu University, Fukuoka 812, Japan.

The effects of synthetic a{alpha}human atrial natriuretic polypeptide ({alpha}hANP) on aldosteronogenesis in normal and aldosterone-producing adenoma cells (APA cells) in primary monolayer cultures were studied. {alpha}hANP significantly inhibited aldosterone secretion from normal adrenal cells in culture, but had no inhibitory effect on aldosterone secretion from APA cells in the presence or absence of 10-8 M ACTH. {alpha}hANP enhanced the accumulation of intracellular cGMP in normal adrenal cells in culture, but not in APA cells. Visualization of [125I]iodoahANP-specific binding sites in APA and adjacent normal adrenal tissues by an in vitro receptor autoradiographic technique showed that these sites were localized only in normal adrenal tissue, but not in APA tissue. These results suggest that the lack of an inhibitory effect of ahANP on aldosteronogenesis in APA cells may be due to the absence of ahANP–specific receptor sites in APA cells.

* This work was suported in part by a Grant 60480273 for research from the Ministry of Education, Japan.

Received October 28, 1985.






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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
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Copyright © 1986 by The Endocrine Society