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Journal of Clinical Endocrinology & Metabolism, Vol 62, 1288-1295, Copyright © 1986 by Endocrine Society


ARTICLES

Electrophoretic analyses of secreted human endometrial proteins: identification and characterization of luteal phase prolactin

LJ Heffner, DA Iddenden and CR Lyttle

Endometrial protein synthesis and secretion throughout the menstrual cycle was studied by slab gel electrophoretic analysis of [35S]methionine incorporation into protein during short term culture of human endometrial tissue. A minimum of five protein bands that fluctuate during the menstrual cycle were identified on one-dimensional gels. Those with mol wt of 28K, 35K, 51K, and 59K decreased in the luteal phase, whereas a broad 25K band was induced in the luteal phase. This broad band was identified as two species of glycosylated PRL by antihuman PRL immunostaining and [3H]glucosamine incorporation.


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Reproductive SciencesHome page
L. J. Heffner, M. Kumari, and L. A. Benoit
Secretion of the Vasoactive Peptides, Endothelin, and Parathyroid Hormone-Related Peptide, by Decidual Explants From Pregnancies Complicated by Intrauterine Growth Restriction
Reproductive Sciences, September 1, 1999; 6(5): 273 - 277.
[Abstract] [PDF]




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