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Gonadotropin-Releasing Hormone Neurosecretion in the Human Hypothalamus: In Vitro Regulation by Dopamine^*

Departments of Reproductive Medicine and Pathology La Jolla, California 92093
University of California, San Diego La Jolla, California 92093

Address requests for reprints to: Dennis Rasmussen, Ph,D., Department of Reproductive Medicine T-002, University of California, San Diego, La Jolla, California 92093.

An in vitro perifusion system was used to investigate GnRH release from adult human hypothalami in response to dopamine (DA) and the DA receptor antagonist haloperidol (HAL). Administration of a 1-µM pulse of DA consistently elicited a mean ± SE 218 ± 59%increase(P < 0.05; n = 5) in GnRH release, whereas 1 µM HAL had no effect. Administration of 1 µM DA during three perifusions in which 1 µM HAL was added to the medium failedto alter basal GnRH release. Incontrast, DA did evoke an acute 98 ± 39% increase (P < 0.06) in GnRH release during threematching perifusions with medium containing the -adrenergic antagonist phentolamine. These studies demonstrate that DA can stimulate in vitro release of GnRH from the adult human hypothalamus by a DA receptormediated mechanism.

^* This work was supported in part by a Mellon Foundation Faculty Scholar Award (to D.D.R.)and NIH Grant HD-12303, and was conducted in part by the Clayton Foundation for Research, California Division (S.S.C.Y).

Received September 6, 1985.

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