Journal of Clinical Endocrinology & Metabolism, Vol 61, 1076-1080, Copyright © 1985 by Endocrine Society

ARTICLES

Endogenous luteinizing hormone surge during superovulation induction with sequential use of clomiphene citrate and pulsatile human menopausal gonadotropin

IE Messinis, A Templeton and DT Baird

There is dispute as to whether an endogenous LH surge occurs in women during follicle stimulation with human menopausal gonadotropin (hMG) for in vitro fertilization (IVF). In this study, 12 consecutive normally cycling women with tubal infertility were treated with clomiphene citrate and hMG for ovulation induction and subsequent IVF. Beginning on the fifth day of clomiphene administration (150 mg daily for 5 days), 225 IU hMG were given daily sc in a pulsatile manner (28 IU/3 h). All women had an endogenous LH surge which started on the seventh to ninth day of hMG administration. The mean duration [25.5 +/- 1.0 (+/- sem) h] and the mean peak value (38.9 +/- 4.7 U/liter) of the LH surge were less than those in spontaneous cycles. However, luteinization of at least some of the follicles occurred, based on findings of supraphysiological levels of serum progesterone during the surge and subsequent increase in urinary pregnanediol levels (luteal phase). Preovulatory oocytes were recovered via laparoscopy 34-35 h after the onset of the surge, and these oocytes cleaved after IVF. One pregnancy was achieved after embryo transfer, which resulted in abortion. These results represent the first demonstration that an attenuated LH surge is obtained in normally cycling women during superovulation induction with sequential clomiphene/pulsatile hMG treatment.

This article has been cited by other articles:

				M. Isikoglu, S. Ozdem, M. Berkkanoglu, H. Jamal, Z. Senturk, and K. Ozgur Single-dose depot leuprolide is as efficient as daily short-acting leuprolide in ICSI cycles Hum. Reprod., June 1, 2007; 22(6): 1657 - 1661. [Abstract] [Full Text] [PDF]

				I. E. MESSINIS From Menarche to Regular Menstruation: Endocrinological Background Ann. N.Y. Acad. Sci., December 1, 2006; 1092(1): 49 - 56. [Abstract] [Full Text] [PDF]

				I. E. Messinis Ovarian feedback, mechanism of action and possible clinical implications Hum. Reprod. Update, September 1, 2006; 12(5): 557 - 571. [Abstract] [Full Text] [PDF]

				E. Karligiotou, P. Kollia, A. Kallitsaris, and I.E. Messinis Expression of human serum albumin (HSA) mRNA in human granulosa cells: potential correlation of the 95 amino acid long carboxyl terminal of HSA to gonadotrophin surge-attenuating factor Hum. Reprod., March 1, 2006; 21(3): 645 - 650. [Abstract] [Full Text] [PDF]

				K. Dafopoulos, I. Mademtzis, P. Vanakara, A. Kallitsaris, G. Stamatiou, C. Kotsovassilis, and I. E. Messinis Evidence That Termination of the Estradiol-Induced Luteinizing Hormone Surge in Women Is Regulated by Ovarian Factors J. Clin. Endocrinol. Metab., February 1, 2006; 91(2): 641 - 645. [Abstract] [Full Text] [PDF]

				I.E. Messinis, D. Loutradis, E. Domali, C.P. Kotsovassilis, L. Papastergiopoulou, A. Kallitsaris, P. Drakakis, K. Dafopoulos, and S. Milingos Alternate day and daily administration of GnRH antagonist may prevent premature luteinization to a similar extent during FSH treatment Hum. Reprod., November 1, 2005; 20(11): 3192 - 3197. [Abstract] [Full Text] [PDF]

				I. E. MESSINIS Modulatory Effect of the Ovary on LH Secretion Ann. N.Y. Acad. Sci., November 1, 2003; 997(1): 35 - 41. [Abstract] [Full Text] [PDF]

				H. J. Ingerslev, A. Hojgaard, J. Hindkjar, and U. Kesmodel A randomized study comparing IVF in the unstimulated cycle with IVF following clomiphene citrate Hum. Reprod., April 1, 2001; 16(4): 696 - 702. [Abstract] [Full Text] [PDF]

				I. E. MESSINIS Ovarian Regulators of Gonadotropin Secretion Ann. N.Y. Acad. Sci., April 1, 2000; 900(1): 10 - 15. [Abstract] [Full Text] [PDF]