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Cleaved Prolactin: Evidence for Its Occurrence in Human Pituitary Gland and Plasma^*

Lutcher Brown Department of Biochemistry, The Whittier Institute for Diabetes and Endocrinology La olla, California 92037
The Department of Reproductive Medicine, University of California-San Diego La Jolla, California 92093

Address requests for reprints to: Dr. Y. N. Sinha, Luther Brown Department of Biochemistry, The Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92037.

A form of PRL that is cleaved in its large disul-fide loop has been reported in rat and mouse pituitary glands, but it is not known whether it exists in the human pituitary gland and whether it circulates in blood. Recently, we developed an immunoelectrophoretic technique which is capable of measuring immunoreactive variants of PRL and other hormones in plasma. We describe here detection of cleaved PRL in normal and tumor pituitary tissue from a prolactinoma patient and in the plasma of pregnant women.

^* This work was supported by PHS Grants CA-33448 and AM-32311.

Received February 10, 1984.

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