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Journal of Clinical Endocrinology & Metabolism, Vol 59, 1133-1137, Copyright © 1984 by Endocrine Society

ARTICLES

The isolation and sequence analysis of vasoactive intestinal peptide from a ganglioneuroblastoma

NW Bunnett, JR Reeve Jr, R Dimaline, JE Shively, D Hawke and JH Walsh

A ganglioneuroblastoma was excised at surgery from a 1-yr-old girl with severe watery diarrhea. The tumor, weighing 1 g, was extracted in trifluoracetic acid and contained 8.3 nmol immunoreactive vasoactive intestinal peptide. The peptide was isolated by affinity chromatography and high pressure liquid chromatography and was found to be identical to porcine vasoactive intestinal peptide by amino acid analysis and microsequence analysis.




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Copyright © 1984 by The Endocrine Society