help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holmes, S. D.
Right arrow Articles by Smith, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holmes, S. D.
Right arrow Articles by Smith, R. G.

Journal of Clinical Endocrinology & Metabolism, Vol 59, 1058-1062, Copyright © 1984 by Endocrine Society

ARTICLES

Regulation of transferrin secretion by human Sertoli cells cultured in the presence or absence of human peritubular cells

SD Holmes, LI Lipshultz and RG Smith

Transferrin represents 1-4% of the total proteins secreted by human Sertoli cells, and the amount secreted by the cells was maximal after a 4- to 5-day period in culture, the time chosen for each medium change. During this first 4- to 5-day period, the addition of FSH, insulin, (Bu)2cAMP, and isobutylmethylxanthine had no effect on transferrin secretion; however, from days 4-5 to 8-10, each of the above compounds significantly stimulated transferrin secretion compared to control values. Testosterone (in the absence or presence of insulin) had no effect. Transferrin secretion increased for the first 5 days in culture, with a similar magnitude in the presence or absence of the above stimulators, and thereafter declined, more so in untreated cultures. These results suggest that these agents do not stimulate, but, rather, limit the decline in transferrin secretion. When human peritubular cells were cocultured with Sertoli cells, transferrin secretion was significantly elevated compared to that by Sertoli cells alone. Interestingly, in the cocultures (Bu)2cAMP stimulated transferrin secretion when added for the first 4- to 5-day culture period. Human fibroblasts or spent medium from the peritubular cell cultures did not mimic the effect found when peritubular and Sertoli cells were cocultured. These results provide evidence that peritubular cells play a critical role in regulating human Sertoli cell function.


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
Y. S. Devi, K. Sarda, B. Stephen, P. Nagarajan, and S. S. Majumdar
Follicle-Stimulating Hormone-Independent Functions of Primate Sertoli Cells: Potential Implications in the Diagnosis and Management of Male Infertility
J. Clin. Endocrinol. Metab., March 1, 2006; 91(3): 1062 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Lecureuil, M.C. Saleh, I. Fontaine, B. Baron, M.M. Zakin, and F. Guillou
Transgenic mice as a model to study the regulation of human transferrin expression in Sertoli cells
Hum. Reprod., June 1, 2004; 19(6): 1300 - 1307.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Lefevre, G. Moutel, F. Carre-Pigeon, J. Caron, and P. Gillery
Automated Nephelometric Assay for Seminal Fluid Transferrin
Clin. Chem., December 1, 1998; 44(12): 2544 - 2546.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1984 by The Endocrine Society