help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Davies, T. F.
Right arrow Articles by Friedman, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Davies, T. F.
Right arrow Articles by Friedman, E.

Journal of Clinical Endocrinology & Metabolism, Vol 57, 1021-1027, Copyright © 1983 by Endocrine Society

ARTICLES

Functionality of thyroid-stimulating antibodies assessed by cryopreserved human thyroid cell bioassay

TF Davies, M Platzer, A Schwartz and E Friedman

Immunoglobulin preparations from patients with autoimmune thyroid disease may inhibit the binding of 125I-labeled bovine TSH to solubilized TSH receptors without exhibiting concurrent thyroid stimulation. Such data have emphasized the continuing need for assay of biological function in addition to binding inhibition. We have employed a convenient technique using normal human thyroid cells cultured in monolayers for 7 days and then cryopreserved in liquid N2. Thyroid stimulation was assessed by measuring intracellular cAMP accumulation in a modified hypotonic Hank's Balanced Salt Solution using approximately 30,000 cells/well. Forty-five consecutive unselected immunoglobulin samples from hyperthyroid Graves' patients were assayed at 10 mg/ml protein for 2 h. Thirty-two (71%) of these untreated samples and 3 of 13 (23%) samples from treated euthyroid Graves' patients caused significantly greater stimulation than normal Ig prepared and assayed simultaneously. Forty-four percent of the positive samples were of low titer, causing less than a 200% increase in basal cAMP levels. One high titer sample (12,000% increase) had even greater bioactivity when serially diluted, thus suggesting the presence of an inhibitor, a phenomenon not observed in 20 lower titer and negative preparations. These data indicate the convenience and usefulness of a cryopreserved thyroid cell bioassay and emphasize that only low titers of thyroid-stimulating antibody occur in the peripheral circulation of many Graves' patients.


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
J. Van Sande, M. J. Costa, C. Massart, S. Swillens, S. Costagliola, J. Orgiazzi, and J. E. Dumont
Kinetics of Thyrotropin-Stimulating Hormone (TSH) and Thyroid-Stimulating Antibody Binding and Action on the TSH Receptor in Intact TSH Receptor-Expressing CHO Cells
J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5366 - 5374.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. F. Davies
Thyroid-Stimulating Antibodies Predict Hyperthyroidisma
J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 3777 - 3781.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1983 by The Endocrine Society