This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by HOFMANN, G. E. Articles by SANFILIPPO, J. S. Search for Related Content PubMed PubMed Citation Articles by HOFMANN, G. E., CH Articles by SANFILIPPO, J. S.

Topography of Human Uterine Prostaglandin E and F₂ Receptors and Their Profiles during Pathological States^*

Departments of Obstetrics/Gynecology (G.E.H., C. V.R., J.S.S.) and Pathology (G.H.B.), University of Louisville School of Medicine Louisville, Kentucky 40202

Address requests for reprints to: Dr. Ch. V. Rao, Department of Obstetrics and Gynecology, 436B MDR Building, University of Louisville School of Medicine, Louisville, Kentucky 40202.

Present address of Dr. Hofmann, Department of Obstetrics and Gynecology, University of Cincinnati School of Medicine, Cincinnati, OH 45267.

The topography of prostaglandin (PG) E and F₂ receptors in uteri of premenopausal women was investigated by dividing uteri into six equal longitudinal strips and further dividing each strip into approximately 1-cm segments. Tissue for determination of smooth muscle content using the Trichrome stain was taken from each section, and the remainder was homogenized for binding studies with ³H-labeled PGs. The [³H] PGE₁ binding (mean, 41.5 fmol/mg protein; range, 23.1–58.3) was about 8-fold greater in the fundus than [³H]PGF₂ binding (mean, 4.8 fmol/mg protein; range, 1.3–13.0), and this trend was found in most uterine sections. The binding of both ³H-labeled PGs decreased from fundus to cervix, and this decrease was similar to the decrease in smooth muscle content. Scatchard analysis revealed apparent dissociation constants (K_ds) of 1.4 and 76 nM and apparent specific binding capacities (Ns) of 25 and 488 fmol/mg protein for [³H]PGE₂, and K_d values of 11.5 and 81 nM and Ns values of 19.4 and 58 fmol/mg protein for [³H]PGF₂ in the uterine fundus. The Kd values for [³H]PGE₂ were similar in other sections of the uterus, but the Ns values were smaller in the lower uterine body and cervical end. While the phase of the menstrual cycle did not influence [³H]PG binding, the diagnosis of abnormal uterine bleeding compared to dysmenorrhea was associated with an increase in [³H]PGE₁ binding (P < 0.05).

^* This work was supported in part by a summer research scholarship (to G.E.H.).

Received December 21, 1982.

This article has been cited by other articles:

				H. N. Jabbour, R. W. Kelly, H. M. Fraser, and H. O. D. Critchley Endocrine Regulation of Menstruation Endocr. Rev., February 1, 2006; 27(1): 17 - 46. [Abstract] [Full Text] [PDF]

				K. J. Sales, T. List, S. C. Boddy, A. R.W. Williams, R. A. Anderson, Z. Naor, and H. N. Jabbour A Novel Angiogenic Role for Prostaglandin F2{alpha}-FP Receptor Interaction in Human Endometrial Adenocarcinomas Cancer Res., September 1, 2005; 65(17): 7707 - 7716. [Abstract] [Full Text] [PDF]

				K. J. Sales, S. Maudsley, and H. N. Jabbour Elevated Prostaglandin EP2 Receptor in Endometrial Adenocarcinoma Cells Promotes Vascular Endothelial Growth Factor Expression via Cyclic 3',5'-Adenosine Monophosphate-Mediated Transactivation of the Epidermal Growth Factor Receptor and Extracellular Signal-Regulated Kinase 1/2 Signaling Pathways Mol. Endocrinol., June 1, 2004; 18(6): 1533 - 1545. [Abstract] [Full Text] [PDF]

				K. J. Sales, S. A. Milne, A. R. W. Williams, R. A. Anderson, and H. N. Jabbour Expression, Localization, and Signaling of Prostaglandin F2{alpha} Receptor in Human Endometrial Adenocarcinoma: Regulation of Proliferation by Activation of the Epidermal Growth Factor Receptor and Mitogen-Activated Protein Kinase Signaling Pathways J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 986 - 993. [Abstract] [Full Text] [PDF]

				G. B. Perchick and H. N. Jabbour Cyclooxygenase-2 Overexpression Inhibits Cathepsin D-Mediated Cleavage of Plasminogen to the Potent Antiangiogenic Factor Angiostatin Endocrinology, December 1, 2003; 144(12): 5322 - 5328. [Abstract] [Full Text] [PDF]